Antinociceptive effect of Nephelium lappaceum L. fruit peel and the participation of nitric oxide, opioid receptors, and ATP-sensitive potassium channels
Introduction:Nephelium lappaceum L. (Sapindaceae) is a plant known as rambutan. It is used for various purposes in traditional medicine.Objective: We aimed to evaluate the antinociceptive effects of the ethanol extract of the fruit peel of N. lappaceum (EENL), the mechanisms involved in these effect...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1287580/full |
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| author | Alan Santos Oliveira Laiza Santos Biano David Nascimento Palmeira Damião Rabelo de Almeida Mônica Lopes-Ferreira Markus Kohlhoff Jordano Augusto Carvalho Sousa Geraldo Célio Brandão Ana Mara de Oliveira e Silva Ana Mara de Oliveira e Silva Renata Grespan Renata Grespan Enilton Aparecido Camargo Enilton Aparecido Camargo Enilton Aparecido Camargo Enilton Aparecido Camargo |
| author_facet | Alan Santos Oliveira Laiza Santos Biano David Nascimento Palmeira Damião Rabelo de Almeida Mônica Lopes-Ferreira Markus Kohlhoff Jordano Augusto Carvalho Sousa Geraldo Célio Brandão Ana Mara de Oliveira e Silva Ana Mara de Oliveira e Silva Renata Grespan Renata Grespan Enilton Aparecido Camargo Enilton Aparecido Camargo Enilton Aparecido Camargo Enilton Aparecido Camargo |
| author_sort | Alan Santos Oliveira |
| collection | DOAJ |
| description | Introduction:Nephelium lappaceum L. (Sapindaceae) is a plant known as rambutan. It is used for various purposes in traditional medicine.Objective: We aimed to evaluate the antinociceptive effects of the ethanol extract of the fruit peel of N. lappaceum (EENL), the mechanisms involved in these effects, and the acute toxicity in zebrafish.Methods: We performed chromatography coupled to mass spectrometry, acute toxicity assay in zebrafish, and evaluation in mice submitted to models of nociception and locomotor activity.Results: We identified (epi)-catechin, procyanidin B, and ellagic acid and its derivatives in EENL. We did not find any toxicity in zebrafish embryos incubated with EENL. The locomotor activity of mice submitted to oral pretreatment with EENL was not changed, but it reduced the abdominal constrictions induced by acetic acid, the licking/biting time in both the first and second phase of formalin testing and capsaicin testing, and carrageenan-induced paw mechanical allodynia. Oral pretreatment with EENL increased latency time in the hot plate test. This antinociceptive effect was significantly reversed by naloxone, L-arginine, and glibenclamide respectively showing the participation of opioid receptors, nitric oxide, and KATP channels as mediators of EENL-induced antinociception.Conclusion: EENL causes antinociception with the participation of opioid receptors, nitric oxide, and KATP channels, and is not toxic to zebrafish. |
| first_indexed | 2024-03-11T14:29:04Z |
| format | Article |
| id | doaj.art-4cbcac3382df4d3ebac2420abfc1735b |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-11T14:29:04Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-4cbcac3382df4d3ebac2420abfc1735b2023-10-31T11:30:17ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-10-011410.3389/fphar.2023.12875801287580Antinociceptive effect of Nephelium lappaceum L. fruit peel and the participation of nitric oxide, opioid receptors, and ATP-sensitive potassium channelsAlan Santos Oliveira0Laiza Santos Biano1David Nascimento Palmeira2Damião Rabelo de Almeida3Mônica Lopes-Ferreira4Markus Kohlhoff5Jordano Augusto Carvalho Sousa6Geraldo Célio Brandão7Ana Mara de Oliveira e Silva8Ana Mara de Oliveira e Silva9Renata Grespan10Renata Grespan11Enilton Aparecido Camargo12Enilton Aparecido Camargo13Enilton Aparecido Camargo14Enilton Aparecido Camargo15Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, BrazilPhysiological Sciences Graduate Program, Federal University of Sergipe, São Cristóvão, BrazilHealth Sciences Graduate Program, Federal University of Sergipe, Aracaju, BrazilDepartment of Physiology, Federal University of Sergipe, São Cristóvão, BrazilImmunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP), São Paulo, BrazilOswaldo Cruz Foundation, René Rachou Institute, Belo Horizonte, BrazilDepartment of Pharmacy, Federal University of Ouro Preto, Ouro Preto, BrazilDepartment of Pharmacy, Federal University of Ouro Preto, Ouro Preto, BrazilHealth Sciences Graduate Program, Federal University of Sergipe, Aracaju, BrazilDepartment of Nutrition, Federal University of Sergipe, São Cristóvão, BrazilPhysiological Sciences Graduate Program, Federal University of Sergipe, São Cristóvão, BrazilDepartment of Nutrition, Federal University of Sergipe, São Cristóvão, BrazilHealth Sciences Graduate Program, Federal University of Sergipe, Aracaju, BrazilPhysiological Sciences Graduate Program, Federal University of Sergipe, São Cristóvão, BrazilDepartment of Physiology, Federal University of Sergipe, São Cristóvão, BrazilDepartment of Nutrition, Federal University of Sergipe, São Cristóvão, BrazilIntroduction:Nephelium lappaceum L. (Sapindaceae) is a plant known as rambutan. It is used for various purposes in traditional medicine.Objective: We aimed to evaluate the antinociceptive effects of the ethanol extract of the fruit peel of N. lappaceum (EENL), the mechanisms involved in these effects, and the acute toxicity in zebrafish.Methods: We performed chromatography coupled to mass spectrometry, acute toxicity assay in zebrafish, and evaluation in mice submitted to models of nociception and locomotor activity.Results: We identified (epi)-catechin, procyanidin B, and ellagic acid and its derivatives in EENL. We did not find any toxicity in zebrafish embryos incubated with EENL. The locomotor activity of mice submitted to oral pretreatment with EENL was not changed, but it reduced the abdominal constrictions induced by acetic acid, the licking/biting time in both the first and second phase of formalin testing and capsaicin testing, and carrageenan-induced paw mechanical allodynia. Oral pretreatment with EENL increased latency time in the hot plate test. This antinociceptive effect was significantly reversed by naloxone, L-arginine, and glibenclamide respectively showing the participation of opioid receptors, nitric oxide, and KATP channels as mediators of EENL-induced antinociception.Conclusion: EENL causes antinociception with the participation of opioid receptors, nitric oxide, and KATP channels, and is not toxic to zebrafish.https://www.frontiersin.org/articles/10.3389/fphar.2023.1287580/fullNephelium lappaceumfruit peelantinociceptionlocomotor activitytoxicity |
| spellingShingle | Alan Santos Oliveira Laiza Santos Biano David Nascimento Palmeira Damião Rabelo de Almeida Mônica Lopes-Ferreira Markus Kohlhoff Jordano Augusto Carvalho Sousa Geraldo Célio Brandão Ana Mara de Oliveira e Silva Ana Mara de Oliveira e Silva Renata Grespan Renata Grespan Enilton Aparecido Camargo Enilton Aparecido Camargo Enilton Aparecido Camargo Enilton Aparecido Camargo Antinociceptive effect of Nephelium lappaceum L. fruit peel and the participation of nitric oxide, opioid receptors, and ATP-sensitive potassium channels Frontiers in Pharmacology Nephelium lappaceum fruit peel antinociception locomotor activity toxicity |
| title | Antinociceptive effect of Nephelium lappaceum L. fruit peel and the participation of nitric oxide, opioid receptors, and ATP-sensitive potassium channels |
| title_full | Antinociceptive effect of Nephelium lappaceum L. fruit peel and the participation of nitric oxide, opioid receptors, and ATP-sensitive potassium channels |
| title_fullStr | Antinociceptive effect of Nephelium lappaceum L. fruit peel and the participation of nitric oxide, opioid receptors, and ATP-sensitive potassium channels |
| title_full_unstemmed | Antinociceptive effect of Nephelium lappaceum L. fruit peel and the participation of nitric oxide, opioid receptors, and ATP-sensitive potassium channels |
| title_short | Antinociceptive effect of Nephelium lappaceum L. fruit peel and the participation of nitric oxide, opioid receptors, and ATP-sensitive potassium channels |
| title_sort | antinociceptive effect of nephelium lappaceum l fruit peel and the participation of nitric oxide opioid receptors and atp sensitive potassium channels |
| topic | Nephelium lappaceum fruit peel antinociception locomotor activity toxicity |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1287580/full |
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