Expression and purification of human diacylglycerol kinase α from baculovirus-infected insect cells for structural studies

Diacylglycerol kinases (DGKs) are lipid kinases that modulate the levels of lipid second messengers, diacylglycerol and phosphatidic acid. Recently, increasing attention has been paid to its α isozyme (DGKα) as a potential target for cancer immunotherapy. DGKα consists of the N-terminal regulatory d...

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Bibliographic Details
Main Authors: Daisuke Takahashi, Fumio Sakane
Format: Article
Language:English
Published: PeerJ Inc. 2018-08-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/5449.pdf