Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture
Summary: Many vaccines, including those using recombinant antigen subunits, rely on adjuvant(s) to enhance the efficacy of the host immune responses. Among the few adjuvants clinically approved, QS-21, a saponin-based immunomodulatory molecule isolated from the tree bark of Quillaja saponaria (QS) i...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422400227X |
| _version_ | 1797317448182005760 |
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| author | Xiangmin Lv Jesse Martin Heather Hoover Bishnu Joshi Margaret Wilkens David A. Ullisch Thomas Leibold John S. Juchum Sanket Revadkar Barbara Kalinovska Justin Keith Adam Truby Gui Liu Elaine Sun John Haserick Jon DeGnore Joseph Conolly Adrian V.S. Hill John Baldoni Charlotte Kensil Dan Levey Alexandra J. Spencer Gilbert Gorr Mark Findeis Antoine Tanne |
| author_facet | Xiangmin Lv Jesse Martin Heather Hoover Bishnu Joshi Margaret Wilkens David A. Ullisch Thomas Leibold John S. Juchum Sanket Revadkar Barbara Kalinovska Justin Keith Adam Truby Gui Liu Elaine Sun John Haserick Jon DeGnore Joseph Conolly Adrian V.S. Hill John Baldoni Charlotte Kensil Dan Levey Alexandra J. Spencer Gilbert Gorr Mark Findeis Antoine Tanne |
| author_sort | Xiangmin Lv |
| collection | DOAJ |
| description | Summary: Many vaccines, including those using recombinant antigen subunits, rely on adjuvant(s) to enhance the efficacy of the host immune responses. Among the few adjuvants clinically approved, QS-21, a saponin-based immunomodulatory molecule isolated from the tree bark of Quillaja saponaria (QS) is used in complex formulations in approved effective vaccines. High demand of the QS raw material as well as manufacturing scalability limitation has been barriers here. We report for the first-time successful plant cell culture production of QS-21 having structural, chemical, and biologic, properties similar to the bark extracted product. These data ensure QS-21 and related saponins are broadly available and accessible to drug developers. |
| first_indexed | 2024-03-08T03:35:05Z |
| format | Article |
| id | doaj.art-4ce190e0d41a4a90adc8bb0b7532bfc5 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T03:35:05Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-4ce190e0d41a4a90adc8bb0b7532bfc52024-02-10T04:45:10ZengElsevieriScience2589-00422024-03-01273109006Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell cultureXiangmin Lv0Jesse Martin1Heather Hoover2Bishnu Joshi3Margaret Wilkens4David A. Ullisch5Thomas Leibold6John S. Juchum7Sanket Revadkar8Barbara Kalinovska9Justin Keith10Adam Truby11Gui Liu12Elaine Sun13John Haserick14Jon DeGnore15Joseph Conolly16Adrian V.S. Hill17John Baldoni18Charlotte Kensil19Dan Levey20Alexandra J. Spencer21Gilbert Gorr22Mark Findeis23Antoine Tanne24Agenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAPhyton Biotech GmbH, Ahrensburg, GermanyPhyton Biotech GmbH, Ahrensburg, GermanyPhyton Biotech LLC, 1503 Cliveden Avenue, Delta, BC V3M 6P7, CanadaAgenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UKSaponiQx, Lexington MA, USAAgenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAAgenus, Inc, Lexington MA, USAThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UKSaponiQx, Lexington MA, USASaponiQx, Lexington MA, USAAgenus, Inc, Lexington MA, USAThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK; Hunter Medical Research Institute, School of Biomedical Sciences and Pharmacy, College of Health, Medicine & Wellbeing; Immune Health Program, New Lambton Heights, NSW, AustraliaPhyton Biotech GmbH, Ahrensburg, GermanyAgenus, Inc, Lexington MA, USASaponiQx, Lexington MA, USA; Corresponding authorSummary: Many vaccines, including those using recombinant antigen subunits, rely on adjuvant(s) to enhance the efficacy of the host immune responses. Among the few adjuvants clinically approved, QS-21, a saponin-based immunomodulatory molecule isolated from the tree bark of Quillaja saponaria (QS) is used in complex formulations in approved effective vaccines. High demand of the QS raw material as well as manufacturing scalability limitation has been barriers here. We report for the first-time successful plant cell culture production of QS-21 having structural, chemical, and biologic, properties similar to the bark extracted product. These data ensure QS-21 and related saponins are broadly available and accessible to drug developers.http://www.sciencedirect.com/science/article/pii/S258900422400227Xnatural product synthesismedical biochemistrycellPhytochemistryBioactive plant product |
| spellingShingle | Xiangmin Lv Jesse Martin Heather Hoover Bishnu Joshi Margaret Wilkens David A. Ullisch Thomas Leibold John S. Juchum Sanket Revadkar Barbara Kalinovska Justin Keith Adam Truby Gui Liu Elaine Sun John Haserick Jon DeGnore Joseph Conolly Adrian V.S. Hill John Baldoni Charlotte Kensil Dan Levey Alexandra J. Spencer Gilbert Gorr Mark Findeis Antoine Tanne Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture iScience natural product synthesis medical biochemistry cell Phytochemistry Bioactive plant product |
| title | Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture |
| title_full | Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture |
| title_fullStr | Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture |
| title_full_unstemmed | Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture |
| title_short | Chemical and biological characterization of vaccine adjuvant QS-21 produced via plant cell culture |
| title_sort | chemical and biological characterization of vaccine adjuvant qs 21 produced via plant cell culture |
| topic | natural product synthesis medical biochemistry cell Phytochemistry Bioactive plant product |
| url | http://www.sciencedirect.com/science/article/pii/S258900422400227X |
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