Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed Repair

Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed Repair

An accurate visual reporter system to assess homology-directed repair (HDR) is a key prerequisite for evaluating the efficiency of Cas9-mediated precise gene editing. Herein, we tested the utility of the widespread promoterless EGFP reporter to assess the efficiency of CRISPR/Cas9-mediated homologou...

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Bibliographic Details
Main Authors: Wanqing Xu, Qingxia Zuo, Dongyan Feng, Changsheng He, Cailing Lin, Dongchao Huang, Yanbin Wan, Feng Chen, Guosheng Mo, Qi Sun, Hongli Du, Lizhen Huang
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Current Issues in Molecular Biology
Subjects:
CRISPR/Cas9
homology-directed repair
precise gene editing
random integration
promoterless EGFP reporter
Online Access:https://www.mdpi.com/1467-3045/44/4/116

Internet

https://www.mdpi.com/1467-3045/44/4/116

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