Unbiased choice of global clustering parameters for single-molecule localization microscopy

Abstract Single-molecule localization microscopy resolves objects below the diffraction limit of light via sparse, stochastic detection of target molecules. Single molecules appear as clustered detection events after image reconstruction. However, identification of clusters of localizations is often...

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Bibliographic Details
Main Authors: Pietro Verzelli, Andreas Nold, Chao Sun, Mike Heilemann, Erin M. Schuman, Tatjana Tchumatchenko
Format: Article
Language:English
Published: Nature Portfolio 2022-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-27074-1