Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin
Sky1 is the only member of the SR (Serine–Arginine) protein kinase family in Saccharomyces cerevisiae. When yeast cells are treated with the anti-cancer drug cisplatin, Sky1 kinase activity is necessary to produce the cytotoxic effect. In this study, proteome changes in response to this drug and/or...
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MDPI AG
2014-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/15/7/12573 |
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| author | Silvia Rodríguez-Lombardero M. Esther Rodríguez-Belmonte M. Isabel González-Siso Ángel Vizoso-Vázquez Vanessa Valdiglesias Blanca Laffón M. Esperanza Cerdán |
| author_facet | Silvia Rodríguez-Lombardero M. Esther Rodríguez-Belmonte M. Isabel González-Siso Ángel Vizoso-Vázquez Vanessa Valdiglesias Blanca Laffón M. Esperanza Cerdán |
| author_sort | Silvia Rodríguez-Lombardero |
| collection | DOAJ |
| description | Sky1 is the only member of the SR (Serine–Arginine) protein kinase family in Saccharomyces cerevisiae. When yeast cells are treated with the anti-cancer drug cisplatin, Sky1 kinase activity is necessary to produce the cytotoxic effect. In this study, proteome changes in response to this drug and/or SKY1 deletion have been evaluated in order to understand the role of Sky1 in the response of yeast cells to cisplatin. Results reveal differential expression of proteins previously related to the oxidative stress response, DNA damage, apoptosis and mitophagy. With these precedents, the role of Sky1 in apoptosis, necrosis and mitophagy has been evaluated by flow-cytometry, fluorescence microscopy, biosensors and fluorescence techniques. After cisplatin treatment, an apoptotic-like process diminishes in the ∆sky1 strain in comparison to the wild-type. The treatment does not affect mitophagy in the wild-type strain, while an increase is observed in the ∆sky1 strain. The increased resistance to cisplatin observed in the ∆sky1 strain may be attributable to a decrease of apoptosis and an increase of mitophagy. |
| first_indexed | 2024-04-11T23:52:56Z |
| format | Article |
| id | doaj.art-4cf88032cbe2417e8033cc2189733cee |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-11T23:52:56Z |
| publishDate | 2014-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4cf88032cbe2417e8033cc2189733cee2022-12-22T03:56:27ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-07-01157125731259010.3390/ijms150712573ijms150712573Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to CisplatinSilvia Rodríguez-Lombardero0M. Esther Rodríguez-Belmonte1M. Isabel González-Siso2Ángel Vizoso-Vázquez3Vanessa Valdiglesias4Blanca Laffón5M. Esperanza Cerdán6EXPRELA Group, Department of Cellular and Molecular Biology, University of A Coruña, Campus A Coruña, A Coruña E15071, SpainEXPRELA Group, Department of Cellular and Molecular Biology, University of A Coruña, Campus A Coruña, A Coruña E15071, SpainEXPRELA Group, Department of Cellular and Molecular Biology, University of A Coruña, Campus A Coruña, A Coruña E15071, SpainEXPRELA Group, Department of Cellular and Molecular Biology, University of A Coruña, Campus A Coruña, A Coruña E15071, SpainDICOMOSA Group, Department of Psychology, Area of Psychobiology, University of A Coruña, Campus A Coruña, A Coruña E15071, SpainDICOMOSA Group, Department of Psychology, Area of Psychobiology, University of A Coruña, Campus A Coruña, A Coruña E15071, SpainEXPRELA Group, Department of Cellular and Molecular Biology, University of A Coruña, Campus A Coruña, A Coruña E15071, SpainSky1 is the only member of the SR (Serine–Arginine) protein kinase family in Saccharomyces cerevisiae. When yeast cells are treated with the anti-cancer drug cisplatin, Sky1 kinase activity is necessary to produce the cytotoxic effect. In this study, proteome changes in response to this drug and/or SKY1 deletion have been evaluated in order to understand the role of Sky1 in the response of yeast cells to cisplatin. Results reveal differential expression of proteins previously related to the oxidative stress response, DNA damage, apoptosis and mitophagy. With these precedents, the role of Sky1 in apoptosis, necrosis and mitophagy has been evaluated by flow-cytometry, fluorescence microscopy, biosensors and fluorescence techniques. After cisplatin treatment, an apoptotic-like process diminishes in the ∆sky1 strain in comparison to the wild-type. The treatment does not affect mitophagy in the wild-type strain, while an increase is observed in the ∆sky1 strain. The increased resistance to cisplatin observed in the ∆sky1 strain may be attributable to a decrease of apoptosis and an increase of mitophagy.http://www.mdpi.com/1422-0067/15/7/12573Bmh1Bmh2chemoresistanceyeastcancer |
| spellingShingle | Silvia Rodríguez-Lombardero M. Esther Rodríguez-Belmonte M. Isabel González-Siso Ángel Vizoso-Vázquez Vanessa Valdiglesias Blanca Laffón M. Esperanza Cerdán Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin International Journal of Molecular Sciences Bmh1 Bmh2 chemoresistance yeast cancer |
| title | Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin |
| title_full | Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin |
| title_fullStr | Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin |
| title_full_unstemmed | Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin |
| title_short | Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin |
| title_sort | proteomic analyses reveal that sky1 modulates apoptosis and mitophagy in saccharomyces cerevisiae cells exposed to cisplatin |
| topic | Bmh1 Bmh2 chemoresistance yeast cancer |
| url | http://www.mdpi.com/1422-0067/15/7/12573 |
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