Isolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803

Isolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803

Summary: The FoF1 ATP synthase (ATPase) is one of the most important protein complexes in energy metabolism. The isolation of functional ATPase complexes is fundamental to address questions about its assembly, regulation, and functions. This protocol describes the purification of intact and active A...

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Bibliographic Details
Main Authors: Kuo Song, Stefan Tholen, Desirée Baumgartner, Oliver Schilling, Wolfgang R. Hess
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Metabolism
Microbiology
Plant sciences
Molecular Biology
Protein Biochemistry
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005032

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http://www.sciencedirect.com/science/article/pii/S2666166722005032

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