Protective Mechanism of <i>Rosa roxburghii</i> Tratt Fermentation Broth against Ultraviolet-A-Induced Photoaging of Human Embryonic Skin Fibroblasts
This study takes the fruit of <i>Rosa roxburghii</i> Tratt (RRT) as a fermentation substrate and carries out a quantitative visual analysis of the domestic and foreign literature on screenings of five different lactic acid bacteria to obtain a fermentation broth. Systemic anti-photoaging...
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MDPI AG
2024-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/13/3/382 |
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| author | Minglu Yuan Hao Fu Qiuting Mo Shiwei Wang Changtao Wang Dongdong Wang Jiachan Zhang Meng Li |
| author_facet | Minglu Yuan Hao Fu Qiuting Mo Shiwei Wang Changtao Wang Dongdong Wang Jiachan Zhang Meng Li |
| author_sort | Minglu Yuan |
| collection | DOAJ |
| description | This study takes the fruit of <i>Rosa roxburghii</i> Tratt (RRT) as a fermentation substrate and carries out a quantitative visual analysis of the domestic and foreign literature on screenings of five different lactic acid bacteria to obtain a fermentation broth. Systemic anti-photoaging effects are analyzed at the biochemical, cellular, and molecular biological levels. DPPH and ABTS free radical scavenging activities are used to verify the antioxidant capacity of the RRT fruit fermentation broth in vitro. Human embryonic skin fibroblasts (HESs) are used to establish a UVA damage model, and the antioxidant capacity of the RRT fruit fermentation broth is verified in terms of intracellular reactive oxygen species (ROS) and antioxidant enzyme activity. RT-qPCR and ELISA are used to detect the expression of TGF-β/Smad, MMPs, and the MAPK/AP-1 and Nrf2/Keap-1 signaling pathways in order to explore the anti-oxidation and anti-photoaging effects of the RRT fruit fermentation broth by regulating different signaling pathways. The results show that an RRT fruit fermentation broth can effectively protect cells from oxidative stress caused by UVA and has significant anti-photoaging effects, with the co-cultured <i>Lactobacillus</i> Yogurt Starter LYS-20297 having the highest overall effect. |
| first_indexed | 2024-04-24T18:36:33Z |
| format | Article |
| id | doaj.art-4d103e25920c47b4820a079edb096506 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-04-24T18:36:33Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4d103e25920c47b4820a079edb0965062024-03-27T13:18:45ZengMDPI AGAntioxidants2076-39212024-03-0113338210.3390/antiox13030382Protective Mechanism of <i>Rosa roxburghii</i> Tratt Fermentation Broth against Ultraviolet-A-Induced Photoaging of Human Embryonic Skin FibroblastsMinglu Yuan0Hao Fu1Qiuting Mo2Shiwei Wang3Changtao Wang4Dongdong Wang5Jiachan Zhang6Meng Li7Key Laboratory of Cosmetic, China National Light Industry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Cosmetic, China National Light Industry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Cosmetic, China National Light Industry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi’an 710069, ChinaKey Laboratory of Cosmetic, China National Light Industry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Cosmetic, China National Light Industry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Cosmetic, China National Light Industry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Cosmetic, China National Light Industry, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaThis study takes the fruit of <i>Rosa roxburghii</i> Tratt (RRT) as a fermentation substrate and carries out a quantitative visual analysis of the domestic and foreign literature on screenings of five different lactic acid bacteria to obtain a fermentation broth. Systemic anti-photoaging effects are analyzed at the biochemical, cellular, and molecular biological levels. DPPH and ABTS free radical scavenging activities are used to verify the antioxidant capacity of the RRT fruit fermentation broth in vitro. Human embryonic skin fibroblasts (HESs) are used to establish a UVA damage model, and the antioxidant capacity of the RRT fruit fermentation broth is verified in terms of intracellular reactive oxygen species (ROS) and antioxidant enzyme activity. RT-qPCR and ELISA are used to detect the expression of TGF-β/Smad, MMPs, and the MAPK/AP-1 and Nrf2/Keap-1 signaling pathways in order to explore the anti-oxidation and anti-photoaging effects of the RRT fruit fermentation broth by regulating different signaling pathways. The results show that an RRT fruit fermentation broth can effectively protect cells from oxidative stress caused by UVA and has significant anti-photoaging effects, with the co-cultured <i>Lactobacillus</i> Yogurt Starter LYS-20297 having the highest overall effect.https://www.mdpi.com/2076-3921/13/3/382<i>Rosa roxburghii</i> Tratt<i>Lactobacillus</i> fermentation brothanti-photoaginghuman embryonic skin fibroblasts |
| spellingShingle | Minglu Yuan Hao Fu Qiuting Mo Shiwei Wang Changtao Wang Dongdong Wang Jiachan Zhang Meng Li Protective Mechanism of <i>Rosa roxburghii</i> Tratt Fermentation Broth against Ultraviolet-A-Induced Photoaging of Human Embryonic Skin Fibroblasts Antioxidants <i>Rosa roxburghii</i> Tratt <i>Lactobacillus</i> fermentation broth anti-photoaging human embryonic skin fibroblasts |
| title | Protective Mechanism of <i>Rosa roxburghii</i> Tratt Fermentation Broth against Ultraviolet-A-Induced Photoaging of Human Embryonic Skin Fibroblasts |
| title_full | Protective Mechanism of <i>Rosa roxburghii</i> Tratt Fermentation Broth against Ultraviolet-A-Induced Photoaging of Human Embryonic Skin Fibroblasts |
| title_fullStr | Protective Mechanism of <i>Rosa roxburghii</i> Tratt Fermentation Broth against Ultraviolet-A-Induced Photoaging of Human Embryonic Skin Fibroblasts |
| title_full_unstemmed | Protective Mechanism of <i>Rosa roxburghii</i> Tratt Fermentation Broth against Ultraviolet-A-Induced Photoaging of Human Embryonic Skin Fibroblasts |
| title_short | Protective Mechanism of <i>Rosa roxburghii</i> Tratt Fermentation Broth against Ultraviolet-A-Induced Photoaging of Human Embryonic Skin Fibroblasts |
| title_sort | protective mechanism of i rosa roxburghii i tratt fermentation broth against ultraviolet a induced photoaging of human embryonic skin fibroblasts |
| topic | <i>Rosa roxburghii</i> Tratt <i>Lactobacillus</i> fermentation broth anti-photoaging human embryonic skin fibroblasts |
| url | https://www.mdpi.com/2076-3921/13/3/382 |
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