Evaluation of viral concentration and extraction methods for SARS-CoV-2 recovery from wastewater using droplet digital and quantitative RT-PCR

Evaluation of viral concentration and extraction methods for SARS-CoV-2 recovery from wastewater using droplet digital and quantitative RT-PCR

The ongoing pandemic caused by the emergence of SARS-CoV-2 has resulted in millions of deaths worldwide despite the various measures announced by the authorities. Wastewater-based epidemiology has the ability to provide a day-to-day estimation of the number of infected people in a fast and cost-effe...

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Bibliographic Details
Main Authors: Lampros Dimitrakopoulos, Aikaterini Kontou, Areti Strati, Aikaterini Galani, Marios Kostakis, Vasileios Kapes, Evrikleia Lianidou, Nikolaos Thomaidis, Athina Markou
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Case Studies in Chemical and Environmental Engineering
Subjects:
SARS-CoV-2
Wastewater-based epidemiology
Viral concentration
Viral RNA extraction
Droplet digital PCR (ddPCR)
Quantitative reverse transcription-polymerase chain reaction (RT-qPCR)
Online Access:http://www.sciencedirect.com/science/article/pii/S2666016422000469

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http://www.sciencedirect.com/science/article/pii/S2666016422000469

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