Practical fluorescence reconstruction microscopy for large samples and low-magnification imaging.

Fluorescence reconstruction microscopy (FRM) describes a class of techniques where transmitted light images are passed into a convolutional neural network that then outputs predicted epifluorescence images. This approach enables many benefits including reduced phototoxicity, freeing up of fluorescen...

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Bibliographic Details
Main Authors: Julienne LaChance, Daniel J Cohen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-12-01
Series:PLoS Computational Biology
Online Access:https://doi.org/10.1371/journal.pcbi.1008443