Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes

<p>Abstract</p> <p>Background</p> <p>Recombinant protein production in <it>Escherichia coli </it>cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the...

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Bibliographic Details
Main Authors: Štrukelj Borut, Slanc Petra, Jevševar Simona, Jalen Špela, Okršlar Veronika, Škulj Mihaela, Menart Viktor
Format: Article
Language:English
Published: BMC 2008-03-01
Series:Microbial Cell Factories
Online Access:http://www.microbialcellfactories.com/content/7/1/6