Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management
Gliomas are one of the prominent cancers of the central nervous system with limited therapeutic modalities. The present investigation evaluated the synergistic effect of paclitaxel (PAX) and resveratrol (RESV)-loaded Soluplus polymeric nanoparticles (PNPs) against glioma cell lines along with in viv...
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MDPI AG
2021-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/19/3210 |
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| author | Talib Hussain Sathishbabu Paranthaman Syed Mohd Danish Rizvi Afrasim Moin Devegowda Vishakante Gowda Gehad Muhammed Subaiea Mukhtar Ansari Abulrahman Sattam Alanazi |
| author_facet | Talib Hussain Sathishbabu Paranthaman Syed Mohd Danish Rizvi Afrasim Moin Devegowda Vishakante Gowda Gehad Muhammed Subaiea Mukhtar Ansari Abulrahman Sattam Alanazi |
| author_sort | Talib Hussain |
| collection | DOAJ |
| description | Gliomas are one of the prominent cancers of the central nervous system with limited therapeutic modalities. The present investigation evaluated the synergistic effect of paclitaxel (PAX) and resveratrol (RESV)-loaded Soluplus polymeric nanoparticles (PNPs) against glioma cell lines along with in vivo pharmacokinetics and brain distribution study. PAX-RESV-loaded PNPs were prepared by the thin film hydration technique and optimized for different dependent and independent variables by using DoE (Design-Expert) software. The in vitro physiochemical characterization of prepared PAX-RESV-loaded PNPs exhibited appropriate particle size, PDI and % encapsulation efficiency. Cytotoxicity assay revealed that PTX-RESV loaded PNPs had a synergistic antitumor efficacy against C6 glioma cells compared with single and combined pure drugs. Finally, the pharmacokinetic and brain distribution studies in mice demonstrated that the PNPs significantly enhanced the bioavailability of PTX-RESV PNPs than pure PAX and RESV. Thus, the study concluded that PAX-RESV PNPs combination could significantly enhance anti-glioma activity, and this could be developed into a potential glioma treatment strategy. |
| first_indexed | 2024-03-10T06:53:57Z |
| format | Article |
| id | doaj.art-4d62594977914cefaf60250f0d41fab4 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T06:53:57Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-4d62594977914cefaf60250f0d41fab42023-11-22T16:37:18ZengMDPI AGPolymers2073-43602021-09-011319321010.3390/polym13193210Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma ManagementTalib Hussain0Sathishbabu Paranthaman1Syed Mohd Danish Rizvi2Afrasim Moin3Devegowda Vishakante Gowda4Gehad Muhammed Subaiea5Mukhtar Ansari6Abulrahman Sattam Alanazi7Department of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Hail 81442, Saudi ArabiaDepartment of Pharmaceutics, JSS College of Pharmacy, Mysuru 570015, IndiaDepartment of Pharmaceutics, College of Pharmacy, University of Hail, Hail 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Hail, Hail 81442, Saudi ArabiaDepartment of Pharmaceutics, JSS College of Pharmacy, Mysuru 570015, IndiaDepartment of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Hail 81442, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, University of Hail, Hail 81442, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, University of Hail, Hail 81442, Saudi ArabiaGliomas are one of the prominent cancers of the central nervous system with limited therapeutic modalities. The present investigation evaluated the synergistic effect of paclitaxel (PAX) and resveratrol (RESV)-loaded Soluplus polymeric nanoparticles (PNPs) against glioma cell lines along with in vivo pharmacokinetics and brain distribution study. PAX-RESV-loaded PNPs were prepared by the thin film hydration technique and optimized for different dependent and independent variables by using DoE (Design-Expert) software. The in vitro physiochemical characterization of prepared PAX-RESV-loaded PNPs exhibited appropriate particle size, PDI and % encapsulation efficiency. Cytotoxicity assay revealed that PTX-RESV loaded PNPs had a synergistic antitumor efficacy against C6 glioma cells compared with single and combined pure drugs. Finally, the pharmacokinetic and brain distribution studies in mice demonstrated that the PNPs significantly enhanced the bioavailability of PTX-RESV PNPs than pure PAX and RESV. Thus, the study concluded that PAX-RESV PNPs combination could significantly enhance anti-glioma activity, and this could be developed into a potential glioma treatment strategy.https://www.mdpi.com/2073-4360/13/19/3210brain cancercombinational therapygliomanano-biotechnologypolymeric nanoparticles |
| spellingShingle | Talib Hussain Sathishbabu Paranthaman Syed Mohd Danish Rizvi Afrasim Moin Devegowda Vishakante Gowda Gehad Muhammed Subaiea Mukhtar Ansari Abulrahman Sattam Alanazi Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management Polymers brain cancer combinational therapy glioma nano-biotechnology polymeric nanoparticles |
| title | Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management |
| title_full | Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management |
| title_fullStr | Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management |
| title_full_unstemmed | Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management |
| title_short | Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management |
| title_sort | fabrication and characterization of paclitaxel and resveratrol loaded soluplus polymeric nanoparticles for improved bbb penetration for glioma management |
| topic | brain cancer combinational therapy glioma nano-biotechnology polymeric nanoparticles |
| url | https://www.mdpi.com/2073-4360/13/19/3210 |
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