Insights into the protective immune response by immunization with full-length recombinant TprK protein: cellular and humoral responses
Abstract Syphilis has resurged in many countries, which has called attention to vaccine development. Based on the immunization-based rabbit model of infection with the Nichols strain, this study explored the protective immune response of a controversial syphilis vaccine candidate, TprK, and found th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-09-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-023-00748-1 |
| _version_ | 1797453938685902848 |
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| author | Dan Liu Rui Chen Yong-Jing Wang Wei Li Li-Li Liu Li-Rong Lin Tian-Ci Yang Man-Li Tong |
| author_facet | Dan Liu Rui Chen Yong-Jing Wang Wei Li Li-Li Liu Li-Rong Lin Tian-Ci Yang Man-Li Tong |
| author_sort | Dan Liu |
| collection | DOAJ |
| description | Abstract Syphilis has resurged in many countries, which has called attention to vaccine development. Based on the immunization-based rabbit model of infection with the Nichols strain, this study explored the protective immune response of a controversial syphilis vaccine candidate, TprK, and found that immunization with full-length rTprK was effective in attenuating lesion development and accelerating lesion resolution, which could reduce the probability of the pathogen spreading to distant tissue sites to prevent the progression of the disease to some extent. Furthermore, the results revealed that immunization with rTprK not only rapidly induced a strong Th1-like cellular response but also elicited a humoral immune response to produce opsonic antibodies to enhance macrophage-mediated opsonophagocytosis. Although complete protection against infection was not achieved, the study provided a comprehensive and in-depth exploration of the immunogenicity of TprK and highlighted the importance of TprK as a promising syphilis vaccine component. |
| first_indexed | 2024-03-09T15:30:06Z |
| format | Article |
| id | doaj.art-4d7117f3421d4be3bf7ea3c0a3b2659a |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-09T15:30:06Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-4d7117f3421d4be3bf7ea3c0a3b2659a2023-11-26T12:16:27ZengNature Portfolionpj Vaccines2059-01052023-09-018111010.1038/s41541-023-00748-1Insights into the protective immune response by immunization with full-length recombinant TprK protein: cellular and humoral responsesDan Liu0Rui Chen1Yong-Jing Wang2Wei Li3Li-Li Liu4Li-Rong Lin5Tian-Ci Yang6Man-Li Tong7Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen UniversityInstitute of Infectious Disease, School of Medicine, Xiamen UniversityInstitute of Infectious Disease, School of Medicine, Xiamen UniversityInstitute of Infectious Disease, School of Medicine, Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen UniversityCenter of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen UniversityAbstract Syphilis has resurged in many countries, which has called attention to vaccine development. Based on the immunization-based rabbit model of infection with the Nichols strain, this study explored the protective immune response of a controversial syphilis vaccine candidate, TprK, and found that immunization with full-length rTprK was effective in attenuating lesion development and accelerating lesion resolution, which could reduce the probability of the pathogen spreading to distant tissue sites to prevent the progression of the disease to some extent. Furthermore, the results revealed that immunization with rTprK not only rapidly induced a strong Th1-like cellular response but also elicited a humoral immune response to produce opsonic antibodies to enhance macrophage-mediated opsonophagocytosis. Although complete protection against infection was not achieved, the study provided a comprehensive and in-depth exploration of the immunogenicity of TprK and highlighted the importance of TprK as a promising syphilis vaccine component.https://doi.org/10.1038/s41541-023-00748-1 |
| spellingShingle | Dan Liu Rui Chen Yong-Jing Wang Wei Li Li-Li Liu Li-Rong Lin Tian-Ci Yang Man-Li Tong Insights into the protective immune response by immunization with full-length recombinant TprK protein: cellular and humoral responses npj Vaccines |
| title | Insights into the protective immune response by immunization with full-length recombinant TprK protein: cellular and humoral responses |
| title_full | Insights into the protective immune response by immunization with full-length recombinant TprK protein: cellular and humoral responses |
| title_fullStr | Insights into the protective immune response by immunization with full-length recombinant TprK protein: cellular and humoral responses |
| title_full_unstemmed | Insights into the protective immune response by immunization with full-length recombinant TprK protein: cellular and humoral responses |
| title_short | Insights into the protective immune response by immunization with full-length recombinant TprK protein: cellular and humoral responses |
| title_sort | insights into the protective immune response by immunization with full length recombinant tprk protein cellular and humoral responses |
| url | https://doi.org/10.1038/s41541-023-00748-1 |
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