Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane

Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane

Genome editing with sequence-specific nucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), is revolutionizing crop improvement. Developing efficient genome-editing protocols for highly polyploid crops, including sugarcane (x = 10–1...

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Bibliographic Details
Main Authors: Ayman Eid, Chakravarthi Mohan, Sara Sanchez, Duoduo Wang, Fredy Altpeter
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Genome Editing
Subjects:
CRISPR/Cas9
genome editing
polyploid
magnesium chelatase
sugarcane
biolistic gene transfer
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2021.654996/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2021.654996/full

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