Dynamics of the cellular metabolome during human cytomegalovirus infection.
Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. Despite this reliance, the effect of viral infection on host cell metabolic composition remains poorly understood. Here we applied liquid chromatography-tandem mass spectrometry to me...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2006-12-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC1698944?pdf=render |
| _version_ | 1818510431578226688 |
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| author | Joshua Munger Sunil U Bajad Hilary A Coller Thomas Shenk Joshua D Rabinowitz |
| author_facet | Joshua Munger Sunil U Bajad Hilary A Coller Thomas Shenk Joshua D Rabinowitz |
| author_sort | Joshua Munger |
| collection | DOAJ |
| description | Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. Despite this reliance, the effect of viral infection on host cell metabolic composition remains poorly understood. Here we applied liquid chromatography-tandem mass spectrometry to measure the levels of 63 different intracellular metabolites at multiple times after human cytomegalovirus (HCMV) infection of human fibroblasts. Parallel microarray analysis provided complementary data on transcriptional regulation of metabolic pathways. As the infection progressed, the levels of metabolites involved in glycolysis, the citric acid cycle, and pyrimidine nucleotide biosynthesis markedly increased. HCMV-induced transcriptional upregulation of specific glycolytic and citric acid cycle enzymes mirrored the increases in metabolite levels. The peak levels of numerous metabolites during infection far exceeded those observed during normal fibroblast growth or quiescence, demonstrating that HCMV markedly disrupts cellular metabolic homeostasis and institutes its own specific metabolic program. |
| first_indexed | 2024-12-10T23:19:52Z |
| format | Article |
| id | doaj.art-4d830864b70c4767921adbd501d0d305 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-10T23:19:52Z |
| publishDate | 2006-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-4d830864b70c4767921adbd501d0d3052022-12-22T01:29:44ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742006-12-01212e13210.1371/journal.ppat.0020132Dynamics of the cellular metabolome during human cytomegalovirus infection.Joshua MungerSunil U BajadHilary A CollerThomas ShenkJoshua D RabinowitzViral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. Despite this reliance, the effect of viral infection on host cell metabolic composition remains poorly understood. Here we applied liquid chromatography-tandem mass spectrometry to measure the levels of 63 different intracellular metabolites at multiple times after human cytomegalovirus (HCMV) infection of human fibroblasts. Parallel microarray analysis provided complementary data on transcriptional regulation of metabolic pathways. As the infection progressed, the levels of metabolites involved in glycolysis, the citric acid cycle, and pyrimidine nucleotide biosynthesis markedly increased. HCMV-induced transcriptional upregulation of specific glycolytic and citric acid cycle enzymes mirrored the increases in metabolite levels. The peak levels of numerous metabolites during infection far exceeded those observed during normal fibroblast growth or quiescence, demonstrating that HCMV markedly disrupts cellular metabolic homeostasis and institutes its own specific metabolic program.http://europepmc.org/articles/PMC1698944?pdf=render |
| spellingShingle | Joshua Munger Sunil U Bajad Hilary A Coller Thomas Shenk Joshua D Rabinowitz Dynamics of the cellular metabolome during human cytomegalovirus infection. PLoS Pathogens |
| title | Dynamics of the cellular metabolome during human cytomegalovirus infection. |
| title_full | Dynamics of the cellular metabolome during human cytomegalovirus infection. |
| title_fullStr | Dynamics of the cellular metabolome during human cytomegalovirus infection. |
| title_full_unstemmed | Dynamics of the cellular metabolome during human cytomegalovirus infection. |
| title_short | Dynamics of the cellular metabolome during human cytomegalovirus infection. |
| title_sort | dynamics of the cellular metabolome during human cytomegalovirus infection |
| url | http://europepmc.org/articles/PMC1698944?pdf=render |
| work_keys_str_mv | AT joshuamunger dynamicsofthecellularmetabolomeduringhumancytomegalovirusinfection AT sunilubajad dynamicsofthecellularmetabolomeduringhumancytomegalovirusinfection AT hilaryacoller dynamicsofthecellularmetabolomeduringhumancytomegalovirusinfection AT thomasshenk dynamicsofthecellularmetabolomeduringhumancytomegalovirusinfection AT joshuadrabinowitz dynamicsofthecellularmetabolomeduringhumancytomegalovirusinfection |