Unlocking Nature’s Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter

Unlocking Nature’s Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter

Abstract Background Komagataella phaffii (a.k.a. Pichia pastoris) harbors a glutamate utilization pathway in which synthesis of glutamate dehydrogenase 2 and phosphoenolpyruvate carboxykinase (PEPCK) is induced by glutamate. Glutamate-inducible synthesis of these enzymes is regulated by Rtg1p, a cyt...

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Bibliographic Details
Main Authors: Neetu Rajak, Trishna Dey, Yash Sharma, Vedanth Bellad, Pundi N. Rangarajan
Format: Article
Language:English
Published: BMC 2024-02-01
Series:Microbial Cell Factories
Subjects:
Komagataella phaffii
PEPCK promoter
Glutamate inducible expression system
Monosodium glutamate
Online Access:https://doi.org/10.1186/s12934-024-02340-1

Internet

https://doi.org/10.1186/s12934-024-02340-1

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