VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells
VivosX is an in vivo disulfide crosslinking approach that utilizes a pair of strategically positioned cysteines on two proteins to probe physical interactions within cells. Histone H2A.Z, which often replaces one or both copies of H2A in nucleosomes downstream of promoters, was used to validate Vivo...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2018-08-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/36654 |