Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow
Atomic force microscopy is employed to study the influence of the motion of a glycerol solution through a coiled (spiral-wound) polymeric communication pipe on the aggregation state of a protein, with the example of a horseradish peroxidase (HRP) enzyme. The measuring cell with the buffered solution...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/2076-3417/10/14/4825 |
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| author | Vadim S. Ziborov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Irina A. Ivanova Anastasia A. Valueva Vadim Yu. Tatur Andrey N. Negodailov Andrei A. Lukyanitsa Yuri D. Ivanov |
| author_facet | Vadim S. Ziborov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Irina A. Ivanova Anastasia A. Valueva Vadim Yu. Tatur Andrey N. Negodailov Andrei A. Lukyanitsa Yuri D. Ivanov |
| author_sort | Vadim S. Ziborov |
| collection | DOAJ |
| description | Atomic force microscopy is employed to study the influence of the motion of a glycerol solution through a coiled (spiral-wound) polymeric communication pipe on the aggregation state of a protein, with the example of a horseradish peroxidase (HRP) enzyme. The measuring cell with the buffered solution of the protein was placed within the experimental setup over the pipe coil, through which glycerol was pumped. It is demonstrated that, in such a system, the flow of a non-aqueous liquid (glycerol) leads to a change in the physicochemical properties of a protein, whose solution was incubated in the measuring cell placed over the coil. Namely, changes in both the adsorbability onto mica and the aggregation state of the model HRP protein were observed. As glycerol-containing liquids are commonly used in biosensor operations, the results reported herein can be useful to the development of biosensor systems, in which polymeric communications are employed in sample delivery and thermal stabilization systems. The data obtained herein can also be of use for the development of specified hydrodynamic models. |
| first_indexed | 2024-03-10T18:29:32Z |
| format | Article |
| id | doaj.art-4dabedef80bc4d7baa7100aa97ddcc3f |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T18:29:32Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4dabedef80bc4d7baa7100aa97ddcc3f2023-11-20T06:42:31ZengMDPI AGApplied Sciences2076-34172020-07-011014482510.3390/app10144825Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol FlowVadim S. Ziborov0Tatyana O. Pleshakova1Ivan D. Shumov2Andrey F. Kozlov3Irina A. Ivanova4Anastasia A. Valueva5Vadim Yu. Tatur6Andrey N. Negodailov7Andrei A. Lukyanitsa8Yuri D. Ivanov9Institute of Biomedical Chemistry, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 119121 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaFoundation of Perspective Technologies and Novations, 115682 Moscow, RussiaInstitute of Biomedical Chemistry, 119121 Moscow, RussiaAtomic force microscopy is employed to study the influence of the motion of a glycerol solution through a coiled (spiral-wound) polymeric communication pipe on the aggregation state of a protein, with the example of a horseradish peroxidase (HRP) enzyme. The measuring cell with the buffered solution of the protein was placed within the experimental setup over the pipe coil, through which glycerol was pumped. It is demonstrated that, in such a system, the flow of a non-aqueous liquid (glycerol) leads to a change in the physicochemical properties of a protein, whose solution was incubated in the measuring cell placed over the coil. Namely, changes in both the adsorbability onto mica and the aggregation state of the model HRP protein were observed. As glycerol-containing liquids are commonly used in biosensor operations, the results reported herein can be useful to the development of biosensor systems, in which polymeric communications are employed in sample delivery and thermal stabilization systems. The data obtained herein can also be of use for the development of specified hydrodynamic models.https://www.mdpi.com/2076-3417/10/14/4825liquid flowatomic force microscopy sensorprotein aggregationglycerolhorseradish peroxidase |
| spellingShingle | Vadim S. Ziborov Tatyana O. Pleshakova Ivan D. Shumov Andrey F. Kozlov Irina A. Ivanova Anastasia A. Valueva Vadim Yu. Tatur Andrey N. Negodailov Andrei A. Lukyanitsa Yuri D. Ivanov Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow Applied Sciences liquid flow atomic force microscopy sensor protein aggregation glycerol horseradish peroxidase |
| title | Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow |
| title_full | Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow |
| title_fullStr | Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow |
| title_full_unstemmed | Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow |
| title_short | Investigation of the Influence of Liquid Motion in a Flow-Based System on an Enzyme Aggregation State with an Atomic Force Microscopy Sensor: The Effect of Glycerol Flow |
| title_sort | investigation of the influence of liquid motion in a flow based system on an enzyme aggregation state with an atomic force microscopy sensor the effect of glycerol flow |
| topic | liquid flow atomic force microscopy sensor protein aggregation glycerol horseradish peroxidase |
| url | https://www.mdpi.com/2076-3417/10/14/4825 |
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