Modulation of Doxorubicin Intracellular Accumulation and Anticancer Activity by Triterpene Glycoside Cucumarioside A<sub>2</sub>-2
The effect of treatment of Ehrlich ascites carcinoma (EAC) cells with multidrug resistance by holothurian triterpene glycoside, cucumarioside A<sub>2</sub>-2 (CA<sub>2</sub>-2) was evaluated. Calcein-AM efflux assay and doxorubicin (DOX) uptake and retention measurement in ca...
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MDPI AG
2019-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/11/597 |
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| author | Ekaterina Menchinskaya Tatiana Gorpenchenko Alexandra Silchenko Sergey Avilov Dmitry Aminin |
| author_facet | Ekaterina Menchinskaya Tatiana Gorpenchenko Alexandra Silchenko Sergey Avilov Dmitry Aminin |
| author_sort | Ekaterina Menchinskaya |
| collection | DOAJ |
| description | The effect of treatment of Ehrlich ascites carcinoma (EAC) cells with multidrug resistance by holothurian triterpene glycoside, cucumarioside A<sub>2</sub>-2 (CA<sub>2</sub>-2) was evaluated. Calcein-AM efflux assay and doxorubicin (DOX) uptake and retention measurement in cancer cells, as well as determination of DOX cytotoxic and anticancer effects were applied. Treatment of EAC cells with CA<sub>2</sub>-2 (0.01−0.1 μM) blocked Calcein-AM and DOX efflux from cancer cells and increased the accumulation and cytotoxicity of DOX in EAC cells. Moreover, pre-treatment of mice with EAC by CA<sub>2</sub>-2 (10 μg/kg/5 days, intraperitoneal injection (<i>i.p.</i>)), then transplantation of tumor cells into fresh animals and subsequent treatment of these mice with DOX (2 mg/kg/3 days <i>i.p.</i>) significantly increased average life span (ALS) of mice bearing a tumor and therefore boosted the antitumor effect of doxorubicin in vivo. |
| first_indexed | 2024-04-11T18:04:06Z |
| format | Article |
| id | doaj.art-4de641e277d04abeb078daf42c597528 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T18:04:06Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4de641e277d04abeb078daf42c5975282022-12-22T04:10:23ZengMDPI AGMarine Drugs1660-33972019-10-01171159710.3390/md17110597md17110597Modulation of Doxorubicin Intracellular Accumulation and Anticancer Activity by Triterpene Glycoside Cucumarioside A<sub>2</sub>-2Ekaterina Menchinskaya0Tatiana Gorpenchenko1Alexandra Silchenko2Sergey Avilov3Dmitry Aminin4G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100-letya Vladivostoka, 159, 690022 Vladivostok, RussiaFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far-Eastern Branch of the Russian Academy of Science, Prospect 100-letya Vladivostoka, 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100-letya Vladivostoka, 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100-letya Vladivostoka, 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100-letya Vladivostoka, 159, 690022 Vladivostok, RussiaThe effect of treatment of Ehrlich ascites carcinoma (EAC) cells with multidrug resistance by holothurian triterpene glycoside, cucumarioside A<sub>2</sub>-2 (CA<sub>2</sub>-2) was evaluated. Calcein-AM efflux assay and doxorubicin (DOX) uptake and retention measurement in cancer cells, as well as determination of DOX cytotoxic and anticancer effects were applied. Treatment of EAC cells with CA<sub>2</sub>-2 (0.01−0.1 μM) blocked Calcein-AM and DOX efflux from cancer cells and increased the accumulation and cytotoxicity of DOX in EAC cells. Moreover, pre-treatment of mice with EAC by CA<sub>2</sub>-2 (10 μg/kg/5 days, intraperitoneal injection (<i>i.p.</i>)), then transplantation of tumor cells into fresh animals and subsequent treatment of these mice with DOX (2 mg/kg/3 days <i>i.p.</i>) significantly increased average life span (ALS) of mice bearing a tumor and therefore boosted the antitumor effect of doxorubicin in vivo.https://www.mdpi.com/1660-3397/17/11/597cucumarioside a<sub>2</sub>-2doxorubicinehrlich ascites carcinomamdrsea cucumberstriterpene glycosides |
| spellingShingle | Ekaterina Menchinskaya Tatiana Gorpenchenko Alexandra Silchenko Sergey Avilov Dmitry Aminin Modulation of Doxorubicin Intracellular Accumulation and Anticancer Activity by Triterpene Glycoside Cucumarioside A<sub>2</sub>-2 Marine Drugs cucumarioside a<sub>2</sub>-2 doxorubicin ehrlich ascites carcinoma mdr sea cucumbers triterpene glycosides |
| title | Modulation of Doxorubicin Intracellular Accumulation and Anticancer Activity by Triterpene Glycoside Cucumarioside A<sub>2</sub>-2 |
| title_full | Modulation of Doxorubicin Intracellular Accumulation and Anticancer Activity by Triterpene Glycoside Cucumarioside A<sub>2</sub>-2 |
| title_fullStr | Modulation of Doxorubicin Intracellular Accumulation and Anticancer Activity by Triterpene Glycoside Cucumarioside A<sub>2</sub>-2 |
| title_full_unstemmed | Modulation of Doxorubicin Intracellular Accumulation and Anticancer Activity by Triterpene Glycoside Cucumarioside A<sub>2</sub>-2 |
| title_short | Modulation of Doxorubicin Intracellular Accumulation and Anticancer Activity by Triterpene Glycoside Cucumarioside A<sub>2</sub>-2 |
| title_sort | modulation of doxorubicin intracellular accumulation and anticancer activity by triterpene glycoside cucumarioside a sub 2 sub 2 |
| topic | cucumarioside a<sub>2</sub>-2 doxorubicin ehrlich ascites carcinoma mdr sea cucumbers triterpene glycosides |
| url | https://www.mdpi.com/1660-3397/17/11/597 |
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