An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum)
Spike density and processing quality are important traits in modern wheat production and are controlled by multiple gene loci. The associated genes have been intensively studied and new discoveries have been constantly reported during the past few decades. However, no gene playing a significant role...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2018-03-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.117.300562 |
| _version_ | 1819106901609152512 |
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| author | Bin-Jie Xu Qing Chen Ting Zheng Yun-Feng Jiang Yuan-Yuan Qiao Zhen-Ru Guo Yong-Li Cao Yan Wang Ya-Zhou Zhang Lu-Juan Zong Jing Zhu Cai-Hong Liu Qian-Tao Jiang Xiu-Jin Lan Jian Ma Ji-Rui Wang You-Liang Zheng Yu-Ming Wei Peng-Fei Qi |
| author_facet | Bin-Jie Xu Qing Chen Ting Zheng Yun-Feng Jiang Yuan-Yuan Qiao Zhen-Ru Guo Yong-Li Cao Yan Wang Ya-Zhou Zhang Lu-Juan Zong Jing Zhu Cai-Hong Liu Qian-Tao Jiang Xiu-Jin Lan Jian Ma Ji-Rui Wang You-Liang Zheng Yu-Ming Wei Peng-Fei Qi |
| author_sort | Bin-Jie Xu |
| collection | DOAJ |
| description | Spike density and processing quality are important traits in modern wheat production and are controlled by multiple gene loci. The associated genes have been intensively studied and new discoveries have been constantly reported during the past few decades. However, no gene playing a significant role in the development of these two traits has been identified. In the current study, a common wheat mutant with extremely compact spikes and good processing quality was isolated and characterized. A new allele (Qc1) of the Q gene (an important domestication gene) responsible for the mutant phenotype was cloned, and the molecular mechanism for the mutant phenotype was studied. Results revealed that Qc1 originated from a point mutation that interferes with the miRNA172-directed cleavage of Q transcripts, leading to its overexpression. It also reduces the longitudinal cell size of rachises, resulting in an increased spike density. Furthermore, Qc1 increases the number of vascular bundles, which suggests a higher efficiency in the transportation of assimilates in the spikes of the mutant than that of wild type. This accounts for the improved processing quality. The effects of Qc1 on spike density and wheat processing quality were confirmed by analyzing nine common wheat mutants possessing four different Qc alleles. These results deepen our understanding of the key roles of Q gene, and provide new insights for the potential application of Qc alleles in wheat quality breeding. |
| first_indexed | 2024-12-22T02:45:30Z |
| format | Article |
| id | doaj.art-4e16ed8794da47b989bfcb1f22d985c4 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-22T02:45:30Z |
| publishDate | 2018-03-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-4e16ed8794da47b989bfcb1f22d985c42022-12-21T18:41:32ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362018-03-018377177810.1534/g3.117.3005623An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum)Bin-Jie XuQing ChenTing ZhengYun-Feng JiangYuan-Yuan QiaoZhen-Ru GuoYong-Li CaoYan WangYa-Zhou ZhangLu-Juan ZongJing ZhuCai-Hong LiuQian-Tao JiangXiu-Jin LanJian MaJi-Rui WangYou-Liang ZhengYu-Ming WeiPeng-Fei QiSpike density and processing quality are important traits in modern wheat production and are controlled by multiple gene loci. The associated genes have been intensively studied and new discoveries have been constantly reported during the past few decades. However, no gene playing a significant role in the development of these two traits has been identified. In the current study, a common wheat mutant with extremely compact spikes and good processing quality was isolated and characterized. A new allele (Qc1) of the Q gene (an important domestication gene) responsible for the mutant phenotype was cloned, and the molecular mechanism for the mutant phenotype was studied. Results revealed that Qc1 originated from a point mutation that interferes with the miRNA172-directed cleavage of Q transcripts, leading to its overexpression. It also reduces the longitudinal cell size of rachises, resulting in an increased spike density. Furthermore, Qc1 increases the number of vascular bundles, which suggests a higher efficiency in the transportation of assimilates in the spikes of the mutant than that of wild type. This accounts for the improved processing quality. The effects of Qc1 on spike density and wheat processing quality were confirmed by analyzing nine common wheat mutants possessing four different Qc alleles. These results deepen our understanding of the key roles of Q gene, and provide new insights for the potential application of Qc alleles in wheat quality breeding.http://g3journal.org/lookup/doi/10.1534/g3.117.300562bread-making qualitycompact spikepoint mutationprotein contentwheat breedingmutant screen report |
| spellingShingle | Bin-Jie Xu Qing Chen Ting Zheng Yun-Feng Jiang Yuan-Yuan Qiao Zhen-Ru Guo Yong-Li Cao Yan Wang Ya-Zhou Zhang Lu-Juan Zong Jing Zhu Cai-Hong Liu Qian-Tao Jiang Xiu-Jin Lan Jian Ma Ji-Rui Wang You-Liang Zheng Yu-Ming Wei Peng-Fei Qi An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum) G3: Genes, Genomes, Genetics bread-making quality compact spike point mutation protein content wheat breeding mutant screen report |
| title | An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum) |
| title_full | An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum) |
| title_fullStr | An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum) |
| title_full_unstemmed | An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum) |
| title_short | An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum) |
| title_sort | overexpressed q allele leads to increased spike density and improved processing quality in common wheat triticum aestivum |
| topic | bread-making quality compact spike point mutation protein content wheat breeding mutant screen report |
| url | http://g3journal.org/lookup/doi/10.1534/g3.117.300562 |
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