Contrasting levels of fructose and urea added to an annual ryegrass based diet: effects on microbial protein synthesis, nutrient digestibility and fermentation parameters in continuous culture fermenters
The objective of this experiment was to evaluate the effects of the addition of crystalline fructose and urea to an annual ryegrassbased diet on microbial protein synthesis, fermentation profile and nutrient apparent digestibility, using continuous culture fermenters. Six fermenters were used in a 3...
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| Format: | Article |
| Language: | English |
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Universidad Nacional de La Pampa
2019-10-01
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| Series: | Semiárida |
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| Online Access: | https://cerac.unlpam.edu.ar/index.php/semiarida/article/view/4084 |
| _version_ | 1818789565934075904 |
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| author | Mariano Alende Gustavo J. Lascano Thomas C. Jenkins John G. Andrae |
| author_facet | Mariano Alende Gustavo J. Lascano Thomas C. Jenkins John G. Andrae |
| author_sort | Mariano Alende |
| collection | DOAJ |
| description | The objective of this experiment was to evaluate the effects of the addition of crystalline fructose and urea to an annual ryegrassbased diet on microbial protein synthesis, fermentation profile and nutrient apparent digestibility, using continuous culture fermenters. Six fermenters were used in a 3 x 2 factorial arrangement with three levels of water soluble carbohydrates (WSC) obtained by crystalline fructose addition (21, 24 and 27 g.100 g DM1; LWSC, MWSC and HWSC, respectively) and two levels of CP obtained by urea addition (14.6 and 18.6 g.100 g DM1, LCP and HCP, respectively). Four 10d periods were ran sequentially (7d for adaptation, 3d for sampling). Microbial protein synthesis was assessed by purine to N ratio. There was a positive interaction between WSC and CP level on microbial protein synthesis (P<0.001). Water soluble carbohydrate level did not affect fermentation pH, ammonia concentration or total volatile fatty acids concentration (VFA). Greater CP levels also increased acetic acid proportion and tended to increase acetic to propionic acid ratio, whereas WSC level did not affect VFA proportions. Treatments did not affect nutrient digestibility. We conclude that the addition of crystalline fructose to annual ryegrass samples increased microbial protein synthesis at the greater levels of CP in diet. |
| first_indexed | 2024-12-18T14:41:35Z |
| format | Article |
| id | doaj.art-4e18e46247824fa8bd650fc50c3e34f2 |
| institution | Directory Open Access Journal |
| issn | 2362-4337 2408-4077 |
| language | English |
| last_indexed | 2024-12-18T14:41:35Z |
| publishDate | 2019-10-01 |
| publisher | Universidad Nacional de La Pampa |
| record_format | Article |
| series | Semiárida |
| spelling | doaj.art-4e18e46247824fa8bd650fc50c3e34f22022-12-21T21:04:22ZengUniversidad Nacional de La PampaSemiárida2362-43372408-40772019-10-0129110.19137/semiarida.2019(01).33-412916Contrasting levels of fructose and urea added to an annual ryegrass based diet: effects on microbial protein synthesis, nutrient digestibility and fermentation parameters in continuous culture fermentersMariano Alende0Gustavo J. Lascano1Thomas C. Jenkins2John G. Andrae3Instituto Nacional de Tecnología Agropecuaria INTA, 6326 Anguil, La Pampa, Argentina Clemson University, Department of Animal and Veterinary Sciences, Clemson, SC 29634Clemson University, Department of Animal and Veterinary Sciences, Clemson, SC 29634Clemson University, Department of Animal and Veterinary Sciences, Clemson, SC 29634Clemson University, Department of Animal and Veterinary Sciences, Clemson, SC 29634The objective of this experiment was to evaluate the effects of the addition of crystalline fructose and urea to an annual ryegrassbased diet on microbial protein synthesis, fermentation profile and nutrient apparent digestibility, using continuous culture fermenters. Six fermenters were used in a 3 x 2 factorial arrangement with three levels of water soluble carbohydrates (WSC) obtained by crystalline fructose addition (21, 24 and 27 g.100 g DM1; LWSC, MWSC and HWSC, respectively) and two levels of CP obtained by urea addition (14.6 and 18.6 g.100 g DM1, LCP and HCP, respectively). Four 10d periods were ran sequentially (7d for adaptation, 3d for sampling). Microbial protein synthesis was assessed by purine to N ratio. There was a positive interaction between WSC and CP level on microbial protein synthesis (P<0.001). Water soluble carbohydrate level did not affect fermentation pH, ammonia concentration or total volatile fatty acids concentration (VFA). Greater CP levels also increased acetic acid proportion and tended to increase acetic to propionic acid ratio, whereas WSC level did not affect VFA proportions. Treatments did not affect nutrient digestibility. We conclude that the addition of crystalline fructose to annual ryegrass samples increased microbial protein synthesis at the greater levels of CP in diet.https://cerac.unlpam.edu.ar/index.php/semiarida/article/view/4084annual ryegrasscontinuous culturecrude proteinmicrobial protein synthesiswater soluble carbohydrate |
| spellingShingle | Mariano Alende Gustavo J. Lascano Thomas C. Jenkins John G. Andrae Contrasting levels of fructose and urea added to an annual ryegrass based diet: effects on microbial protein synthesis, nutrient digestibility and fermentation parameters in continuous culture fermenters Semiárida annual ryegrass continuous culture crude protein microbial protein synthesis water soluble carbohydrate |
| title | Contrasting levels of fructose and urea added to an annual ryegrass based diet: effects on microbial protein synthesis, nutrient digestibility and fermentation parameters in continuous culture fermenters |
| title_full | Contrasting levels of fructose and urea added to an annual ryegrass based diet: effects on microbial protein synthesis, nutrient digestibility and fermentation parameters in continuous culture fermenters |
| title_fullStr | Contrasting levels of fructose and urea added to an annual ryegrass based diet: effects on microbial protein synthesis, nutrient digestibility and fermentation parameters in continuous culture fermenters |
| title_full_unstemmed | Contrasting levels of fructose and urea added to an annual ryegrass based diet: effects on microbial protein synthesis, nutrient digestibility and fermentation parameters in continuous culture fermenters |
| title_short | Contrasting levels of fructose and urea added to an annual ryegrass based diet: effects on microbial protein synthesis, nutrient digestibility and fermentation parameters in continuous culture fermenters |
| title_sort | contrasting levels of fructose and urea added to an annual ryegrass based diet effects on microbial protein synthesis nutrient digestibility and fermentation parameters in continuous culture fermenters |
| topic | annual ryegrass continuous culture crude protein microbial protein synthesis water soluble carbohydrate |
| url | https://cerac.unlpam.edu.ar/index.php/semiarida/article/view/4084 |
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