Enhancement of a recombinase-aided amplification assay using betaine and pullulan

Enhancement of a recombinase-aided amplification assay using betaine and pullulan

Background: Nucleic acid amplification enhancers suitable for use in a recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) was initially explored. Methods: Using recombinant plasmids and clinical samples, RAA fluorescence and bas...

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Bibliographic Details
Main Authors: Jinrong Wang, Guowei Song, Yue Ming, Jing Pan, Ruiqing Zhang, Guohao Fan, Xinxin Shen, Xuejun Ma, Lixin Li
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:Infectious Medicine
Subjects:
Nucleic acid amplification enhancer
Betaine
Pullulan
RAA
Long-fragment
Online Access:http://www.sciencedirect.com/science/article/pii/S2772431X22000211

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http://www.sciencedirect.com/science/article/pii/S2772431X22000211

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