Enhancement of a recombinase-aided amplification assay using betaine and pullulan
Background: Nucleic acid amplification enhancers suitable for use in a recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) was initially explored. Methods: Using recombinant plasmids and clinical samples, RAA fluorescence and bas...
Main Authors: | Jinrong Wang, Guowei Song, Yue Ming, Jing Pan, Ruiqing Zhang, Guohao Fan, Xinxin Shen, Xuejun Ma, Lixin Li |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-06-01
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Series: | Infectious Medicine |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2772431X22000211 |
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