Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan

Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan

Cell-free system has emerged as a powerful platform with a wide range of in vitro applications and recently has contributed to express metabolic pathways for biosynthesis. Here we report in vitro construction of a native biosynthetic pathway for L-4-nitrotryptophan (L-4-nitro-Trp) synthesis using an...

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Bibliographic Details
Main Authors: Xintong Tian, Wan-Qiu Liu, Huiling Xu, Xiangyang Ji, Yushi Liu, Jian Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2022-06-01
Series:Synthetic and Systems Biotechnology
Subjects:
Cell-free protein synthesis
NO synthase
P450 enzyme
L-4-nitrotryptophan
In vitro biosynthesis
Synthetic biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X22000308

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http://www.sciencedirect.com/science/article/pii/S2405805X22000308

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