Microbial Population Succession and Community Diversity and Its Correlation with Fermentation Quality in Soybean Meal Treated with <i>Enterococcus faecalis</i> during Fermentation and Aerobic Exposure
This study assessed the effects of <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) in combination with protease on fermentation characteristics and microbial communities during ensiling and aerobic exposure phases of soybean meal (SBM). In this study, response surface metho...
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/3/530 |
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| author | Hao Ma Weiwei Wang Zhenyu Wang Zhongfang Tan Guangyong Qin Yanping Wang Huili Pang |
| author_facet | Hao Ma Weiwei Wang Zhenyu Wang Zhongfang Tan Guangyong Qin Yanping Wang Huili Pang |
| author_sort | Hao Ma |
| collection | DOAJ |
| description | This study assessed the effects of <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) in combination with protease on fermentation characteristics and microbial communities during ensiling and aerobic exposure phases of soybean meal (SBM). In this study, response surface methodology (RSM) was used to optimize the optimal growth conditions of <i>E. faecalis</i> ZZUPF95, which produced protease, and fermented SBM under the optimal fermentation conditions. The fermentation test was divided into four groups as follows: CK (Control check), ZZUPF95, Protease and ZZUPF95+Protease groups. Results showed that the best medium ratio of ZZUPF95 was glucose 1%, peptone 2%, inorganic salt 1.47%; fermentation time 36 h, inoculation amount 10%, ratio of material to water 1:1 is the optimal fermentation scheme; after fermentation and aerobic exposure treatment, ZZUPF95 and ZZUPF95 + Protease group can reduce the pH of feed, improve the content of lactic acid in the fermentation system, and have the effect of inhibiting the reproduction of pathogenic bacteria, increasing the content of crude protein and ether extract, and degrading crude fiber; the microbial community of SBM were changed after fermentation and aerobic exposure. This study explored the changes of fermentation quality of SBM, which has certain theoretical value to improve the fermentation mode and storage of SBM. |
| first_indexed | 2024-03-09T13:16:20Z |
| format | Article |
| id | doaj.art-4e2d8ebc784a4aef88202bace8d92d0b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:20Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-4e2d8ebc784a4aef88202bace8d92d0b2023-11-30T21:35:35ZengMDPI AGMicroorganisms2076-26072022-02-0110353010.3390/microorganisms10030530Microbial Population Succession and Community Diversity and Its Correlation with Fermentation Quality in Soybean Meal Treated with <i>Enterococcus faecalis</i> during Fermentation and Aerobic ExposureHao Ma0Weiwei Wang1Zhenyu Wang2Zhongfang Tan3Guangyong Qin4Yanping Wang5Huili Pang6School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, ChinaSchool of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, ChinaHenan Key Lab Ion Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, ChinaHenan Key Lab Ion Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, ChinaHenan Key Lab Ion Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, ChinaHenan Key Lab Ion Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, ChinaHenan Key Lab Ion Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, ChinaThis study assessed the effects of <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) in combination with protease on fermentation characteristics and microbial communities during ensiling and aerobic exposure phases of soybean meal (SBM). In this study, response surface methodology (RSM) was used to optimize the optimal growth conditions of <i>E. faecalis</i> ZZUPF95, which produced protease, and fermented SBM under the optimal fermentation conditions. The fermentation test was divided into four groups as follows: CK (Control check), ZZUPF95, Protease and ZZUPF95+Protease groups. Results showed that the best medium ratio of ZZUPF95 was glucose 1%, peptone 2%, inorganic salt 1.47%; fermentation time 36 h, inoculation amount 10%, ratio of material to water 1:1 is the optimal fermentation scheme; after fermentation and aerobic exposure treatment, ZZUPF95 and ZZUPF95 + Protease group can reduce the pH of feed, improve the content of lactic acid in the fermentation system, and have the effect of inhibiting the reproduction of pathogenic bacteria, increasing the content of crude protein and ether extract, and degrading crude fiber; the microbial community of SBM were changed after fermentation and aerobic exposure. This study explored the changes of fermentation quality of SBM, which has certain theoretical value to improve the fermentation mode and storage of SBM.https://www.mdpi.com/2076-2607/10/3/530optimizationsoybean mealfermentationmicrobial community |
| spellingShingle | Hao Ma Weiwei Wang Zhenyu Wang Zhongfang Tan Guangyong Qin Yanping Wang Huili Pang Microbial Population Succession and Community Diversity and Its Correlation with Fermentation Quality in Soybean Meal Treated with <i>Enterococcus faecalis</i> during Fermentation and Aerobic Exposure Microorganisms optimization soybean meal fermentation microbial community |
| title | Microbial Population Succession and Community Diversity and Its Correlation with Fermentation Quality in Soybean Meal Treated with <i>Enterococcus faecalis</i> during Fermentation and Aerobic Exposure |
| title_full | Microbial Population Succession and Community Diversity and Its Correlation with Fermentation Quality in Soybean Meal Treated with <i>Enterococcus faecalis</i> during Fermentation and Aerobic Exposure |
| title_fullStr | Microbial Population Succession and Community Diversity and Its Correlation with Fermentation Quality in Soybean Meal Treated with <i>Enterococcus faecalis</i> during Fermentation and Aerobic Exposure |
| title_full_unstemmed | Microbial Population Succession and Community Diversity and Its Correlation with Fermentation Quality in Soybean Meal Treated with <i>Enterococcus faecalis</i> during Fermentation and Aerobic Exposure |
| title_short | Microbial Population Succession and Community Diversity and Its Correlation with Fermentation Quality in Soybean Meal Treated with <i>Enterococcus faecalis</i> during Fermentation and Aerobic Exposure |
| title_sort | microbial population succession and community diversity and its correlation with fermentation quality in soybean meal treated with i enterococcus faecalis i during fermentation and aerobic exposure |
| topic | optimization soybean meal fermentation microbial community |
| url | https://www.mdpi.com/2076-2607/10/3/530 |
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