Medium throughput protocol for genome-based quantification of intracellular mycobacterial loads and macrophage survival during in vitro infection

Medium throughput protocol for genome-based quantification of intracellular mycobacterial loads and macrophage survival during in vitro infection

Summary: Here, we present a streamlined protocol for assessing intracellular Mycobacterium tuberculosis (Mtb) loads in macrophages. This protocol describes the simultaneous assessment of macrophage viability using automated microscopy. Further, we detail the quantification of mycobacterial loads usi...

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Bibliographic Details
Main Authors: Shivraj M. Yabaji, Sujoy Chatterjee, Emily Waligursky, Alexander Gimelbrant, Igor Kramnik
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell-based Assays
Immunology
Microbiology
Microscopy
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001216

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http://www.sciencedirect.com/science/article/pii/S2666166722001216

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