High-throughput formation and image-based analysis of basal-in mammary organoids in 384-well plates

Abstract This manuscript describes a new method for forming basal-in MCF10A organoids using commercial 384-well ultra-low attachment (ULA) microplates and the development of associated live-cell imaging and automated analysis protocols. The use of a commercial 384-well ULA platform makes this method...

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Bibliographic Details
Main Authors: Soojung Lee, Jonathan Chang, Sung-Min Kang, Eric Parigoris, Ji-Hoon Lee, Yun Suk Huh, Shuichi Takayama
Format: Article
Language:English
Published: Nature Portfolio 2022-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-03739-1