Multiple Occurrences of a 168-Nucleotide Deletion in SARS-CoV-2 ORF8, Unnoticed by Standard Amplicon Sequencing and Variant Calling Pipelines

Multiple Occurrences of a 168-Nucleotide Deletion in SARS-CoV-2 ORF8, Unnoticed by Standard Amplicon Sequencing and Variant Calling Pipelines

Genomic surveillance of the SARS-CoV-2 pandemic is crucial and mainly achieved by amplicon sequencing protocols. Overlapping tiled-amplicons are generated to establish contiguous SARS-CoV-2 genome sequences, which enable the precise resolution of infection chains and outbreaks. We investigated a SAR...

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Bibliographic Details
Main Authors: David Brandt, Marina Simunovic, Tobias Busche, Markus Haak, Peter Belmann, Sebastian Jünemann, Tizian Schulz, Levin Joe Klages, Svenja Vinke, Michael Beckstette, Ehmke Pohl, Christiane Scherer, Alexander Sczyrba, Jörn Kalinowski
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Viruses
Subjects:
genomic surveillance
SARS-CoV-2
nanopore sequencing
ORF8 deletion
viral genomics
Online Access:https://www.mdpi.com/1999-4915/13/9/1870

Internet

https://www.mdpi.com/1999-4915/13/9/1870

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