Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene <i>Ara h 2</i>

Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene <i>Ara h 2</i>

The cultivated peanut (<i>Arachis hypogaea</i> L.) is a legume consumed worldwide in the form of oil, nuts, peanut butter, and candy. Improving peanut production and nutrition will require new technologies to enable novel trait development. Clustered regularly interspaced short palindrom...

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Bibliographic Details
Main Authors: Sudip Biswas, Nancy J. Wahl, Michael J. Thomson, John M. Cason, Bill F. McCutchen, Endang M. Septiningsih
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:International Journal of Molecular Sciences
Subjects:
peanut (<i>Arachis hypogaea</i> L.)
gene editing
CRISPR–Cas9
<i>Ara h 2</i>
protoplast
transformation efficiency
Online Access:https://www.mdpi.com/1422-0067/23/2/837

Internet

https://www.mdpi.com/1422-0067/23/2/837

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