A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response.
Alphaviruses are RNA viruses transmitted between vertebrate hosts by arthropod vectors, primarily mosquitoes. How arthropods counteract alphaviruses or viruses per se is not very well understood. Drosophila melanogaster is a powerful model system for studying innate immunity against bacterial and fu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-09-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC2738967?pdf=render |
| _version_ | 1818529474245820416 |
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| author | Vasanthi Avadhanula Brandon P Weasner Gail G Hardy Justin P Kumar Richard W Hardy |
| author_facet | Vasanthi Avadhanula Brandon P Weasner Gail G Hardy Justin P Kumar Richard W Hardy |
| author_sort | Vasanthi Avadhanula |
| collection | DOAJ |
| description | Alphaviruses are RNA viruses transmitted between vertebrate hosts by arthropod vectors, primarily mosquitoes. How arthropods counteract alphaviruses or viruses per se is not very well understood. Drosophila melanogaster is a powerful model system for studying innate immunity against bacterial and fungal infections. In this study we report the use of a novel system to analyze replication of Sindbis virus (type species of the alphavirus genus) RNA following expression of a Sindbis virus replicon RNA from the fly genome. We demonstrate deficits in the immune deficiency (Imd) pathway enhance viral replication while mutations in the Toll pathway fail to affect replication. Similar results were observed with intrathoracic injections of whole virus and confirmed in cultured mosquito cells. These findings show that the Imd pathway mediates an antiviral response to Sindbis virus replication. To our knowledge, this is the first demonstration of an antiviral role for the Imd pathway in insects. |
| first_indexed | 2024-12-11T17:07:20Z |
| format | Article |
| id | doaj.art-4e73b32220af49a181a8bece5e4fd797 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-11T17:07:20Z |
| publishDate | 2009-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-4e73b32220af49a181a8bece5e4fd7972022-12-22T00:57:38ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742009-09-0159e100058210.1371/journal.ppat.1000582A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response.Vasanthi AvadhanulaBrandon P WeasnerGail G HardyJustin P KumarRichard W HardyAlphaviruses are RNA viruses transmitted between vertebrate hosts by arthropod vectors, primarily mosquitoes. How arthropods counteract alphaviruses or viruses per se is not very well understood. Drosophila melanogaster is a powerful model system for studying innate immunity against bacterial and fungal infections. In this study we report the use of a novel system to analyze replication of Sindbis virus (type species of the alphavirus genus) RNA following expression of a Sindbis virus replicon RNA from the fly genome. We demonstrate deficits in the immune deficiency (Imd) pathway enhance viral replication while mutations in the Toll pathway fail to affect replication. Similar results were observed with intrathoracic injections of whole virus and confirmed in cultured mosquito cells. These findings show that the Imd pathway mediates an antiviral response to Sindbis virus replication. To our knowledge, this is the first demonstration of an antiviral role for the Imd pathway in insects.http://europepmc.org/articles/PMC2738967?pdf=render |
| spellingShingle | Vasanthi Avadhanula Brandon P Weasner Gail G Hardy Justin P Kumar Richard W Hardy A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response. PLoS Pathogens |
| title | A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response. |
| title_full | A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response. |
| title_fullStr | A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response. |
| title_full_unstemmed | A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response. |
| title_short | A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response. |
| title_sort | novel system for the launch of alphavirus rna synthesis reveals a role for the imd pathway in arthropod antiviral response |
| url | http://europepmc.org/articles/PMC2738967?pdf=render |
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