CATALASE AND PEROXIDASE CONTRIBUTION TO PROMOTING COLD TOLERANCE IN WHEAT

<span>The dynamics of catalase (CaT) and guaiacol peroxidase (POD) activity in winter wheat <span>(<span><em>Triticum aestivum </em><span>L.) leaves under exposure to a low hardening temperature of 4<span>°<span>C for <span>7 days was studied. It...

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Bibliographic Details
Main Authors: Anna Ignatenko, Natalia Repkina, Vera Talanova
Format: Article
Language:English
Published: Karelian Research Centre of the Russian Academy of Sciences 2018-04-01
Series:Transactions of the Karelian Research Centre of the Russian Academy of Sciences
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Online Access:http://journals.krc.karelia.ru/index.php/thematic/article/view/804
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Summary:<span>The dynamics of catalase (CaT) and guaiacol peroxidase (POD) activity in winter wheat <span>(<span><em>Triticum aestivum </em><span>L.) leaves under exposure to a low hardening temperature of 4<span>°<span>C for <span>7 days was studied. It was established that the freezing tolerance of the seedlings increased already after 1 hour of exposure, and then continued to grow, reaching a maximum on the 7<span>th <span>day. The content of malondialdehyde increased slightly during the first <span>2 days of cold exposure and then declined, possibly pointing to attenuation of the oxidative stress in plant cells. It was also found that the activity of one of the key antioxi<span>dant enzymes involved in hydrogen peroxide (h<span>2<span>O<span>2<span>) recycling – CaT, increased after<br /><span>1 hour of cold exposure. CaT activity then continued to increase, reaching a maximum <span>on the 2<span>nd <span>day, with a decline following afterwards. along with this, POD activity increased <span>throughout the cold hardening period. In addition, a slight increase in H<span>2<span>O<span>2 <span>content <span>in leaves was detected during the first 24 hours of cold exposure, but then H<span>2<span>O<span>2 <span>level <span>in the cells decreased towards the end of chilling. Based on these data we can conclude <span>that the increase in the activity of antioxidant enzymes plays an important role in promoting the tolerance to hypothermia in wheat. CaT and POD are involved in h<span>2<span>O<span>2 <span>recycling at<br /><span>the initial stage of low-temperature adaptation in the plants, whereas later on POD plays <span>the main role in this process. Thus, the activation of antioxidant enzymes in leaves prevents the development of oxidative stress and promotes the tolerance of low temperature <span>in wheat.</span></span></span></span></span></span></span></span></span><br /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br /></span></span></span></span></span></span></span>
ISSN:1997-3217
2312-4504