Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP

Summary: Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here, we describe a protocol to monitor actin filament re...

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Bibliographic Details
Main Authors: Ria Fajarwati Kastian, Takunori Minegishi, Naoyuki Inagaki
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721006109