Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
Summary: Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here, we describe a protocol to monitor actin filament re...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721006109 |
| _version_ | 1818873802268868608 |
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| author | Ria Fajarwati Kastian Takunori Minegishi Naoyuki Inagaki |
| author_facet | Ria Fajarwati Kastian Takunori Minegishi Naoyuki Inagaki |
| author_sort | Ria Fajarwati Kastian |
| collection | DOAJ |
| description | Summary: Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here, we describe a protocol to monitor actin filament retrograde flow and actin polymerization within spines using a standard epi-fluorescence microscope. In combination with chemical long-term potentiation, this protocol allows us to quantify clutch coupling efficiency and actin polymerization rate, which are essential variables for generating forces for activity-dependent spine enlargement.For complete details on the use and execution of this protocol, please refer to Kastian et al. (2021). |
| first_indexed | 2024-12-19T13:00:29Z |
| format | Article |
| id | doaj.art-4eca825c5e5a46dbb99d08be8e497bdc |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-19T13:00:29Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-4eca825c5e5a46dbb99d08be8e497bdc2022-12-21T20:20:15ZengElsevierSTAR Protocols2666-16672021-12-0124100904Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTPRia Fajarwati Kastian0Takunori Minegishi1Naoyuki Inagaki2Laboratory of Systems Neurobiology and Medicine, Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan; Corresponding authorLaboratory of Systems Neurobiology and Medicine, Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan; Corresponding authorLaboratory of Systems Neurobiology and Medicine, Division of Biological Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan; Corresponding authorSummary: Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here, we describe a protocol to monitor actin filament retrograde flow and actin polymerization within spines using a standard epi-fluorescence microscope. In combination with chemical long-term potentiation, this protocol allows us to quantify clutch coupling efficiency and actin polymerization rate, which are essential variables for generating forces for activity-dependent spine enlargement.For complete details on the use and execution of this protocol, please refer to Kastian et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721006109Cell BiologyCell cultureMicroscopyNeuroscience |
| spellingShingle | Ria Fajarwati Kastian Takunori Minegishi Naoyuki Inagaki Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP STAR Protocols Cell Biology Cell culture Microscopy Neuroscience |
| title | Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP |
| title_full | Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP |
| title_fullStr | Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP |
| title_full_unstemmed | Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP |
| title_short | Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP |
| title_sort | simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical ltp |
| topic | Cell Biology Cell culture Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721006109 |
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