Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
Summary: Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here, we describe a protocol to monitor actin filament re...
Main Authors: | Ria Fajarwati Kastian, Takunori Minegishi, Naoyuki Inagaki |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-12-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721006109 |
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