Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing

Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing

Abstract Background Current library preparation protocols for Illumina HiSeq and MiSeq DNA sequencers require ≥2 nM initial library for subsequent loading of denatured cDNA onto flow cells. Such amounts are not always attainable from samples having a relatively low DNA or RNA input; or those for whi...

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Bibliographic Details
Main Authors: Wells W. Wu, Je-Nie Phue, Chun-Ting Lee, Changyi Lin, Lai Xu, Rong Wang, Yaqin Zhang, Rong-Fong Shen
Format: Article
Language:English
Published: BMC 2018-05-01
Series:BMC Genomics
Subjects:
Next generation sequencing
Illumina
MiSeq
HiSeq
Sub-nanomolar libraries
Online Access:http://link.springer.com/article/10.1186/s12864-018-4677-y

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http://link.springer.com/article/10.1186/s12864-018-4677-y

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