Terminalin from African Mango (<i>Irvingia gabonensis</i>) Stimulates Glucose Uptake through Inhibition of Protein Tyrosine Phosphatases
Protein tyrosine phosphatases (PTPs), along with protein tyrosine kinases, control signaling pathways involved in cell growth, metabolism, differentiation, proliferation, and survival. Several PTPs, such as PTPN1, PTPN2, PTPN9, PTPN11, PTPRS, and DUSP9, disrupt insulin signaling and trigger type 2 d...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/2/321 |
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| author | Sun-Young Yoon Jinsoo Kim Bum Soo Lee Su Cheol Baek Sang J. Chung Ki Hyun Kim |
| author_facet | Sun-Young Yoon Jinsoo Kim Bum Soo Lee Su Cheol Baek Sang J. Chung Ki Hyun Kim |
| author_sort | Sun-Young Yoon |
| collection | DOAJ |
| description | Protein tyrosine phosphatases (PTPs), along with protein tyrosine kinases, control signaling pathways involved in cell growth, metabolism, differentiation, proliferation, and survival. Several PTPs, such as PTPN1, PTPN2, PTPN9, PTPN11, PTPRS, and DUSP9, disrupt insulin signaling and trigger type 2 diabetes, indicating that PTPs are promising drug targets for the treatment or prevention of type 2 diabetes. As part of an ongoing study on the discovery of pharmacologically active bioactive natural products, we conducted a phytochemical investigation of African mango (<i>Irvingia gabonensis</i>) using liquid chromatography–mass spectrometry (LC/MS)-based analysis, which led to the isolation of terminalin as a major component from the extract of the seeds of <i>I. gabonensis</i>. The structure of terminalin was characterized by spectroscopic methods, including one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) and high-resolution (HR) electrospray ionization (ESI) mass spectroscopy. Moreover, terminalin was evaluated for its antidiabetic property; terminalin inhibited the catalytic activity of PTPN1, PTPN9, PTPN11, and PTPRS in vitro and led to a significant increase in glucose uptake in differentiated C2C12 muscle cells, indicating that terminalin exhibits antidiabetic effect through the PTP inhibitory mechanism. These findings suggest that terminalin derived from African mango could be used as a functional food ingredient or pharmaceutical supplement for the prevention of type 2 diabetes. |
| first_indexed | 2024-03-09T22:28:50Z |
| format | Article |
| id | doaj.art-4ed271b680b1431e89d177e61c5fd28c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T22:28:50Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-4ed271b680b1431e89d177e61c5fd28c2023-11-23T19:00:03ZengMDPI AGBiomolecules2218-273X2022-02-0112232110.3390/biom12020321Terminalin from African Mango (<i>Irvingia gabonensis</i>) Stimulates Glucose Uptake through Inhibition of Protein Tyrosine PhosphatasesSun-Young Yoon0Jinsoo Kim1Bum Soo Lee2Su Cheol Baek3Sang J. Chung4Ki Hyun Kim5Department of Cosmetic Science, Kwangju Women’s University, Gwangju 62396, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaProtein tyrosine phosphatases (PTPs), along with protein tyrosine kinases, control signaling pathways involved in cell growth, metabolism, differentiation, proliferation, and survival. Several PTPs, such as PTPN1, PTPN2, PTPN9, PTPN11, PTPRS, and DUSP9, disrupt insulin signaling and trigger type 2 diabetes, indicating that PTPs are promising drug targets for the treatment or prevention of type 2 diabetes. As part of an ongoing study on the discovery of pharmacologically active bioactive natural products, we conducted a phytochemical investigation of African mango (<i>Irvingia gabonensis</i>) using liquid chromatography–mass spectrometry (LC/MS)-based analysis, which led to the isolation of terminalin as a major component from the extract of the seeds of <i>I. gabonensis</i>. The structure of terminalin was characterized by spectroscopic methods, including one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) and high-resolution (HR) electrospray ionization (ESI) mass spectroscopy. Moreover, terminalin was evaluated for its antidiabetic property; terminalin inhibited the catalytic activity of PTPN1, PTPN9, PTPN11, and PTPRS in vitro and led to a significant increase in glucose uptake in differentiated C2C12 muscle cells, indicating that terminalin exhibits antidiabetic effect through the PTP inhibitory mechanism. These findings suggest that terminalin derived from African mango could be used as a functional food ingredient or pharmaceutical supplement for the prevention of type 2 diabetes.https://www.mdpi.com/2218-273X/12/2/321protein tyrosine phosphatases (PTPs)PTPN1PTPN9PTPN11PTPRStype 2 diabetes |
| spellingShingle | Sun-Young Yoon Jinsoo Kim Bum Soo Lee Su Cheol Baek Sang J. Chung Ki Hyun Kim Terminalin from African Mango (<i>Irvingia gabonensis</i>) Stimulates Glucose Uptake through Inhibition of Protein Tyrosine Phosphatases Biomolecules protein tyrosine phosphatases (PTPs) PTPN1 PTPN9 PTPN11 PTPRS type 2 diabetes |
| title | Terminalin from African Mango (<i>Irvingia gabonensis</i>) Stimulates Glucose Uptake through Inhibition of Protein Tyrosine Phosphatases |
| title_full | Terminalin from African Mango (<i>Irvingia gabonensis</i>) Stimulates Glucose Uptake through Inhibition of Protein Tyrosine Phosphatases |
| title_fullStr | Terminalin from African Mango (<i>Irvingia gabonensis</i>) Stimulates Glucose Uptake through Inhibition of Protein Tyrosine Phosphatases |
| title_full_unstemmed | Terminalin from African Mango (<i>Irvingia gabonensis</i>) Stimulates Glucose Uptake through Inhibition of Protein Tyrosine Phosphatases |
| title_short | Terminalin from African Mango (<i>Irvingia gabonensis</i>) Stimulates Glucose Uptake through Inhibition of Protein Tyrosine Phosphatases |
| title_sort | terminalin from african mango i irvingia gabonensis i stimulates glucose uptake through inhibition of protein tyrosine phosphatases |
| topic | protein tyrosine phosphatases (PTPs) PTPN1 PTPN9 PTPN11 PTPRS type 2 diabetes |
| url | https://www.mdpi.com/2218-273X/12/2/321 |
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