Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches

Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches

Abstract We present a novel genome-wide off-target prediction method named Extru-seq and compare it with cell-based (GUIDE-seq), in vitro (Digenome-seq), and in silico methods using promiscuous guide RNAs with large numbers of valid off-target sites. Extru-seq demonstrates a high validation rate and...

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Bibliographic Details
Main Authors: Jeonghun Kwon, Minyoung Kim, Woochang Hwang, Anna Jo, Gue-Ho Hwang, Minhee Jung, Un Gi Kim, Gang Cui, Heonseok Kim, Joon-Ho Eom, Junho K. Hur, Junwon Lee, Youngho Kim, Jin-soo Kim, Sangsu Bae, Jungjoon K. Lee
Format: Article
Language:English
Published: BMC 2023-01-01
Series:Genome Biology
Subjects:
CRISPR
Genome-wide
Off-target
Cell-based
In vitro
Online Access:https://doi.org/10.1186/s13059-022-02842-4

Internet

https://doi.org/10.1186/s13059-022-02842-4

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