Protocol for Xenium spatial transcriptomics studies using fixed frozen mouse brain sections

Protocol for Xenium spatial transcriptomics studies using fixed frozen mouse brain sections

Summary: Here, we present a protocol for Xenium spatial transcriptomics studies using fixed frozen mouse brain sections. We describe steps for intracardiac perfusion, cryosectioning, and floating section mounting of brain sections, which enable runs on the Xenium analyzer and data delivery. We demon...

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Bibliographic Details
Main Authors: Xiaokuang Ma, Peng Chen, Jing Wei, John Zhang, Chang Chen, Hanqiu Zhao, Deveroux Ferguson, Aaron W. McGee, Zhiyu Dai, Shenfeng Qiu
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
RNA-seq
molecular biology
gene expression
neuroscience
systems biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005859

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http://www.sciencedirect.com/science/article/pii/S2666166724005859

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