In Vivo Validation of a Nanostructured Electrospun Polycaprolactone Membrane Loaded with Gentamicin and Nano-Hydroxyapatite for the Treatment of Periodontitis
The aim of this research was to validate the use of a gentamicin (GEN) and nano-hydroxiapatite (nHAP)-loaded polycaprolactone nanostructured membrane (NM) as an innovative, highly efficient, low-cost treatment for periodontitis. We conducted an in vivo study on Wistar rats, in which we induced perio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/14/3/60 |
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| author | Patricia Ondine Lucaciu Călin Cosmin Repciuc Ioana A. Matei Nicodim I. Fiț Sanda Andrei Raluca Marica Bianca Nausica Petrescu Bogdan Crișan Ovidiu Aghiorghiesei Ioana Codruța Mirică Dragoș Apostu Codruța Saroși Florin Onișor Evelyn Vanea Simina Angela Lăcrimioara Iușan Giorgiana Corina Mureșan Ana-Maria Condor Emilia Oprița Luciana-Mădălina Gherman |
| author_facet | Patricia Ondine Lucaciu Călin Cosmin Repciuc Ioana A. Matei Nicodim I. Fiț Sanda Andrei Raluca Marica Bianca Nausica Petrescu Bogdan Crișan Ovidiu Aghiorghiesei Ioana Codruța Mirică Dragoș Apostu Codruța Saroși Florin Onișor Evelyn Vanea Simina Angela Lăcrimioara Iușan Giorgiana Corina Mureșan Ana-Maria Condor Emilia Oprița Luciana-Mădălina Gherman |
| author_sort | Patricia Ondine Lucaciu |
| collection | DOAJ |
| description | The aim of this research was to validate the use of a gentamicin (GEN) and nano-hydroxiapatite (nHAP)-loaded polycaprolactone nanostructured membrane (NM) as an innovative, highly efficient, low-cost treatment for periodontitis. We conducted an in vivo study on Wistar rats, in which we induced periodontitis by placing silk ligatures around the first right and left upper molars. The subjects were divided into three groups; the first group received no periodontal treatment, the second group received open flap debridement, and the third group received open flap debridement, together with the positioning of the GEN and nHAP-loaded nanostructured membrane as a treatment. The extent of periodontal regeneration was assessed by the periodontal pocket depth, bleeding on probing, tooth mobility, dental plaque, microbiological analysis, concentration of MMP-8 in saliva, plasma levels of CRP, and histological analysis. The results showed that using open flap debridement with the NM is more efficient, and it significantly reduces the probing depth, extent of bleeding on probing, dental mobility, bacterial plaque, and pathogenic flora. The concentrations of MMP-8 and CRP decrease. The histological analysis demonstrated that NM leads to bone regeneration. Our study indicates that gentamicin and nano-hydroxyapatite embedded in the fiber of the biodegradable membranes might be a promising therapeutic option for periodontitis treatment. |
| first_indexed | 2024-04-24T18:01:27Z |
| format | Article |
| id | doaj.art-4ee7424a38ea470c970befcce0dec6cd |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-04-24T18:01:27Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-4ee7424a38ea470c970befcce0dec6cd2024-03-27T13:54:00ZengMDPI AGMembranes2077-03752024-02-011436010.3390/membranes14030060In Vivo Validation of a Nanostructured Electrospun Polycaprolactone Membrane Loaded with Gentamicin and Nano-Hydroxyapatite for the Treatment of PeriodontitisPatricia Ondine Lucaciu0Călin Cosmin Repciuc1Ioana A. Matei2Nicodim I. Fiț3Sanda Andrei4Raluca Marica5Bianca Nausica Petrescu6Bogdan Crișan7Ovidiu Aghiorghiesei8Ioana Codruța Mirică9Dragoș Apostu10Codruța Saroși11Florin Onișor12Evelyn Vanea13Simina Angela Lăcrimioara Iușan14Giorgiana Corina Mureșan15Ana-Maria Condor16Emilia Oprița17Luciana-Mădălina Gherman18Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaDepartment of Surgery Anesthesiology and Intensive Care, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Microbiology, Immunology and Epidemiology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Microbiology, Immunology and Epidemiology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Microbiology, Immunology and Epidemiology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Microbiology, Immunology and Epidemiology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaDepartament of Maxillofacial Surgery and Oral Implantology, Iuliu Hatieganu University of Medicine and Pharmacy, Cardinal Iuliu Hossu nr. 37, 400012 Cluj-Napoca, RomaniaDepartment of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaDepartment of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaDepartament of Orthopaedics and Traumatology, Iuliu Hatieganu University of Medicine and Pharmacy, Traian Mosoiu nr. 47–49, 400012 Cluj-Napoca, RomaniaInstitute of Chemistry Raluca Ripan, Department of Polymer Composites, Babes-Bolyai University, 400294 Cluj-Napoca, RomaniaDepartament of Maxillofacial Surgery and Oral Implantology, Iuliu Hatieganu University of Medicine and Pharmacy, Cardinal Iuliu Hossu nr. 37, 400012 Cluj-Napoca, RomaniaDepartment of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaDepartment of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaDepartment of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaDepartment of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaDepartment of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, Str. Victor Babes 15, 400012 Cluj-Napoca, RomaniaExperimental Centre of University of Medicine and Pharmacy Iuliu Hatieganu, 400349 Cluj-Napoca, RomaniaThe aim of this research was to validate the use of a gentamicin (GEN) and nano-hydroxiapatite (nHAP)-loaded polycaprolactone nanostructured membrane (NM) as an innovative, highly efficient, low-cost treatment for periodontitis. We conducted an in vivo study on Wistar rats, in which we induced periodontitis by placing silk ligatures around the first right and left upper molars. The subjects were divided into three groups; the first group received no periodontal treatment, the second group received open flap debridement, and the third group received open flap debridement, together with the positioning of the GEN and nHAP-loaded nanostructured membrane as a treatment. The extent of periodontal regeneration was assessed by the periodontal pocket depth, bleeding on probing, tooth mobility, dental plaque, microbiological analysis, concentration of MMP-8 in saliva, plasma levels of CRP, and histological analysis. The results showed that using open flap debridement with the NM is more efficient, and it significantly reduces the probing depth, extent of bleeding on probing, dental mobility, bacterial plaque, and pathogenic flora. The concentrations of MMP-8 and CRP decrease. The histological analysis demonstrated that NM leads to bone regeneration. Our study indicates that gentamicin and nano-hydroxyapatite embedded in the fiber of the biodegradable membranes might be a promising therapeutic option for periodontitis treatment.https://www.mdpi.com/2077-0375/14/3/60periodontitismembranegentamicinnano-hydroxyapatitedrug delivery system |
| spellingShingle | Patricia Ondine Lucaciu Călin Cosmin Repciuc Ioana A. Matei Nicodim I. Fiț Sanda Andrei Raluca Marica Bianca Nausica Petrescu Bogdan Crișan Ovidiu Aghiorghiesei Ioana Codruța Mirică Dragoș Apostu Codruța Saroși Florin Onișor Evelyn Vanea Simina Angela Lăcrimioara Iușan Giorgiana Corina Mureșan Ana-Maria Condor Emilia Oprița Luciana-Mădălina Gherman In Vivo Validation of a Nanostructured Electrospun Polycaprolactone Membrane Loaded with Gentamicin and Nano-Hydroxyapatite for the Treatment of Periodontitis Membranes periodontitis membrane gentamicin nano-hydroxyapatite drug delivery system |
| title | In Vivo Validation of a Nanostructured Electrospun Polycaprolactone Membrane Loaded with Gentamicin and Nano-Hydroxyapatite for the Treatment of Periodontitis |
| title_full | In Vivo Validation of a Nanostructured Electrospun Polycaprolactone Membrane Loaded with Gentamicin and Nano-Hydroxyapatite for the Treatment of Periodontitis |
| title_fullStr | In Vivo Validation of a Nanostructured Electrospun Polycaprolactone Membrane Loaded with Gentamicin and Nano-Hydroxyapatite for the Treatment of Periodontitis |
| title_full_unstemmed | In Vivo Validation of a Nanostructured Electrospun Polycaprolactone Membrane Loaded with Gentamicin and Nano-Hydroxyapatite for the Treatment of Periodontitis |
| title_short | In Vivo Validation of a Nanostructured Electrospun Polycaprolactone Membrane Loaded with Gentamicin and Nano-Hydroxyapatite for the Treatment of Periodontitis |
| title_sort | in vivo validation of a nanostructured electrospun polycaprolactone membrane loaded with gentamicin and nano hydroxyapatite for the treatment of periodontitis |
| topic | periodontitis membrane gentamicin nano-hydroxyapatite drug delivery system |
| url | https://www.mdpi.com/2077-0375/14/3/60 |
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