RNA-guided retargeting of Sleeping Beauty transposition in human cells
An ideal tool for gene therapy would enable efficient gene integration at predetermined sites in the human genome. Here we demonstrate biased genome-wide integration of the Sleeping Beauty (SB) transposon by combining it with components of the CRISPR/Cas9 system. We provide proof-of-concept that it...
| Автори: | , , , , , |
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| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
eLife Sciences Publications Ltd
2020-03-01
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| Серія: | eLife |
| Предмети: | |
| Онлайн доступ: | https://elifesciences.org/articles/53868 |