Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays

Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays

Summary: This protocol describes the detailed procedures for utilizing human pluripotent stem cells (hPSCs) for pancreatic progenitor and hepatic differentiation, followed by the application of hPSC-derived cells in a luciferase reporter-based assay to study gene regulation. The generated hPSC-deriv...

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Bibliographic Details
Main Authors: Lay Shuen Tan, Resilind Su Ern Chew, Natasha Hui Jin Ng, Adrian Kee Keong Teo
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell culture
Cell-based Assays
Molecular Biology
Gene Expression
Stem Cells
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001787

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http://www.sciencedirect.com/science/article/pii/S2666166721001787

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