In-Depth Comparison of Adeno-Associated Virus Containing Fractions after CsCl Ultracentrifugation Gradient Separation

In-Depth Comparison of Adeno-Associated Virus Containing Fractions after CsCl Ultracentrifugation Gradient Separation

Recombinant adeno-associated viruses (rAAVs) play a pivotal role in the treatment of genetic diseases. However, current production and purification processes yield AAV-based preparations that often contain unwanted empty, partially filled or damaged viral particles and impurities, including residual...

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Bibliographic Details
Main Authors: Mojca Janc, Kaja Zevnik, Ana Dolinar, Tjaša Jakomin, Maja Štalekar, Katarina Bačnik, Denis Kutnjak, Magda Tušek Žnidarič, Lorena Zentilin, Dmitrii Fedorov, David Dobnik
Format: Article
Language:English
Published: MDPI AG 2024-07-01
Series:Viruses
Subjects:
recombinant adeno-associated viruses (rAAVs)
CsCl ultracentrifugation gradient
analytical methods
digital droplet PCR (ddPCR)
transmission electron microscopy (TEM)
analytical ultracentrifugation (AUC)
Online Access:https://www.mdpi.com/1999-4915/16/8/1235

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https://www.mdpi.com/1999-4915/16/8/1235

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