Nonlinear Structured Illumination Using a Fluorescent Protein Activating at the Readout Wavelength.

Structured illumination microscopy (SIM) is a wide-field technique in fluorescence microscopy that provides fast data acquisition and two-fold resolution improvement beyond the Abbe limit. We observed a further resolution improvement using the nonlinear emission response of a fluorescent protein. We...

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Bibliographic Details
Main Authors: Hui-Wen Lu-Walther, Wenya Hou, Martin Kielhorn, Yoshiyuki Arai, Takeharu Nagai, Michael M Kessels, Britta Qualmann, Rainer Heintzmann
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5082623?pdf=render