Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances
This study aimed to analyze the genetic diversity of selected cultivars and clones of <i>Lonicera</i> sp. (edible honeysuckle) from two countries with different climatic and soil conditions. Accordingly, the determination of total polyphenols content (TPC) and total antioxidant activity...
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MDPI AG
2020-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/6/868 |
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| author | Marcela Cehula Jiří Mlček Tünde Juríková Jana Žiarovská Oleg Paulen Libor Dokoupil Anna Adámková Ramona Babosova |
| author_facet | Marcela Cehula Jiří Mlček Tünde Juríková Jana Žiarovská Oleg Paulen Libor Dokoupil Anna Adámková Ramona Babosova |
| author_sort | Marcela Cehula |
| collection | DOAJ |
| description | This study aimed to analyze the genetic diversity of selected cultivars and clones of <i>Lonicera</i> sp. (edible honeysuckle) from two countries with different climatic and soil conditions. Accordingly, the determination of total polyphenols content (TPC) and total antioxidant activity (TAA) in fruits of <i>Lonicera</i> sp. in 2018–2019 was performed. Applied statistical methods evaluating TPC and TAA were: correlation and regression, ANOVA, and agglomerative hierarchical clustering. The neighbor-joining method and gel images analysis (PyElph software, Pavel and Vasile, Bucharest, Romania) were used for genetic analysis. The average TPC of all cultivars and clones was 5.32 mg L<sup>−1</sup> in the year 2018 and 3.78 mg L<sup>−1</sup> in 2019. The average TAA values of all samples in 2018 were 8.24 mg L<sup>−1</sup>, and 9.27 mg L<sup>−1</sup> in 2019. Statistically significant correlations between years and cultivars and clones were found. Based on the lengths and number of fragments by random amplified polymorphic DNA (RAPD)-PCR’s, it was found that ‘clone 5’ and ‘clone 7’ were genetically close to the grown cultivars from the Czech Republic. Therefore, edible honeysuckle fruits are a valuable source of important nutrients for the human body and its health. |
| first_indexed | 2024-03-10T19:04:04Z |
| format | Article |
| id | doaj.art-4fac6920a5f84f76a70c07299783f0a1 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T19:04:04Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-4fac6920a5f84f76a70c07299783f0a12023-11-20T04:14:53ZengMDPI AGAgronomy2073-43952020-06-0110686810.3390/agronomy10060868Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive SubstancesMarcela Cehula0Jiří Mlček1Tünde Juríková2Jana Žiarovská3Oleg Paulen4Libor Dokoupil5Anna Adámková6Ramona Babosova7Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nábrežie mládeže 91, 949 74 Nitra, SlovakiaDepartment of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 760 01 Zlín, Czech RepublicInstitute for Teacher Training, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Dražovská 4, 949 74 Nitra, SlovakiaDepartment of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Trieda A. Hlinku 2, 949 76 Nitra, SlovakiaHorticulture and Landscape Engineering Faculty, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicDepartment of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 760 01 Zlín, Czech RepublicDepartment of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nábrežie mládeže 91, 949 74 Nitra, SlovakiaThis study aimed to analyze the genetic diversity of selected cultivars and clones of <i>Lonicera</i> sp. (edible honeysuckle) from two countries with different climatic and soil conditions. Accordingly, the determination of total polyphenols content (TPC) and total antioxidant activity (TAA) in fruits of <i>Lonicera</i> sp. in 2018–2019 was performed. Applied statistical methods evaluating TPC and TAA were: correlation and regression, ANOVA, and agglomerative hierarchical clustering. The neighbor-joining method and gel images analysis (PyElph software, Pavel and Vasile, Bucharest, Romania) were used for genetic analysis. The average TPC of all cultivars and clones was 5.32 mg L<sup>−1</sup> in the year 2018 and 3.78 mg L<sup>−1</sup> in 2019. The average TAA values of all samples in 2018 were 8.24 mg L<sup>−1</sup>, and 9.27 mg L<sup>−1</sup> in 2019. Statistically significant correlations between years and cultivars and clones were found. Based on the lengths and number of fragments by random amplified polymorphic DNA (RAPD)-PCR’s, it was found that ‘clone 5’ and ‘clone 7’ were genetically close to the grown cultivars from the Czech Republic. Therefore, edible honeysuckle fruits are a valuable source of important nutrients for the human body and its health.https://www.mdpi.com/2073-4395/10/6/868honeysuckleDNA polymorphismTAATPC |
| spellingShingle | Marcela Cehula Jiří Mlček Tünde Juríková Jana Žiarovská Oleg Paulen Libor Dokoupil Anna Adámková Ramona Babosova Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances Agronomy honeysuckle DNA polymorphism TAA TPC |
| title | Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances |
| title_full | Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances |
| title_fullStr | Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances |
| title_full_unstemmed | Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances |
| title_short | Assessment of Genetic Diversity of Edible Honeysuckle Monitored through RAPD in Relative to Bioactive Substances |
| title_sort | assessment of genetic diversity of edible honeysuckle monitored through rapd in relative to bioactive substances |
| topic | honeysuckle DNA polymorphism TAA TPC |
| url | https://www.mdpi.com/2073-4395/10/6/868 |
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