Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines
Mouse blastocysts contain the committed precursors of the extraembryonic endoderm (ExEn), which express the key transcription factor Oct4, depend on LIF/LIF-like factor-driven Jak/Stat signaling, and initially exhibit lineage plasticity. Previously described rat blastocyst-derived ExEn precursor-lik...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-07-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506118301260 |
| _version_ | 1828337518809972736 |
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| author | Yixiang Zhong Taewoong Choi Minjae Kim Kyoung Hwa Jung Young Gyu Chai Bert Binas |
| author_facet | Yixiang Zhong Taewoong Choi Minjae Kim Kyoung Hwa Jung Young Gyu Chai Bert Binas |
| author_sort | Yixiang Zhong |
| collection | DOAJ |
| description | Mouse blastocysts contain the committed precursors of the extraembryonic endoderm (ExEn), which express the key transcription factor Oct4, depend on LIF/LIF-like factor-driven Jak/Stat signaling, and initially exhibit lineage plasticity. Previously described rat blastocyst-derived ExEn precursor-like cell lines (XENP cells/HypoSCs) also show these features, but equivalent mouse blastocyst-derived cell lines are lacking. We now present mouse blastocyst-derived cell lines, named primitive XEN (pXEN) cells, which share these and additional characteristics with the XENP cells/HypoSCs, but not with previously known mouse blastocyst-derived XEN cell lines. Otherwise, pXEN cells are highly similar to XEN cells by morphology, lineage-intrinsic differentiation potential, and multi-gene expression profile, although the pXEN cell profile correlates better with the blastocyst stage. Finally, we show that pXEN cells easily convert into XEN-like cells but not vice versa. The findings indicate that (i) pXEN cells are more representative than XEN cells of the blastocyst stage; (ii) mouse pXEN, rather than XEN, cells are homologs of rat XENP cells/HypoSCs, which we propose to call rat pXEN cells. Keywords: Stem cells, Blastocyst, Mice, Rats, Extraembryonic endoderm |
| first_indexed | 2024-04-13T22:16:23Z |
| format | Article |
| id | doaj.art-4fb4f202f8244bc4b0881fdac94c16d6 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-13T22:16:23Z |
| publishDate | 2018-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-4fb4f202f8244bc4b0881fdac94c16d62022-12-22T02:27:31ZengElsevierStem Cell Research1873-50612018-07-0130100112Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell linesYixiang Zhong0Taewoong Choi1Minjae Kim2Kyoung Hwa Jung3Young Gyu Chai4Bert Binas5Department of Molecular & Life Science, College of Science and Technology, Hanyang University (ERICA Campus), 55 Hanyangdaehak-ro, Sangrok-gu, Ansan-si, Gyeonggi-do 15588, Republic of Korea.Department of Molecular & Life Science, College of Science and Technology, Hanyang University (ERICA Campus), 55 Hanyangdaehak-ro, Sangrok-gu, Ansan-si, Gyeonggi-do 15588, Republic of Korea.Department of Molecular & Life Science, College of Science and Technology, Hanyang University (ERICA Campus), 55 Hanyangdaehak-ro, Sangrok-gu, Ansan-si, Gyeonggi-do 15588, Republic of Korea.Department of Molecular & Life Science, College of Science and Technology, Hanyang University (ERICA Campus), 55 Hanyangdaehak-ro, Sangrok-gu, Ansan-si, Gyeonggi-do 15588, Republic of Korea.Department of Molecular & Life Science, College of Science and Technology, Hanyang University (ERICA Campus), 55 Hanyangdaehak-ro, Sangrok-gu, Ansan-si, Gyeonggi-do 15588, Republic of Korea.Corresponding author.; Department of Molecular & Life Science, College of Science and Technology, Hanyang University (ERICA Campus), 55 Hanyangdaehak-ro, Sangrok-gu, Ansan-si, Gyeonggi-do 15588, Republic of Korea.Mouse blastocysts contain the committed precursors of the extraembryonic endoderm (ExEn), which express the key transcription factor Oct4, depend on LIF/LIF-like factor-driven Jak/Stat signaling, and initially exhibit lineage plasticity. Previously described rat blastocyst-derived ExEn precursor-like cell lines (XENP cells/HypoSCs) also show these features, but equivalent mouse blastocyst-derived cell lines are lacking. We now present mouse blastocyst-derived cell lines, named primitive XEN (pXEN) cells, which share these and additional characteristics with the XENP cells/HypoSCs, but not with previously known mouse blastocyst-derived XEN cell lines. Otherwise, pXEN cells are highly similar to XEN cells by morphology, lineage-intrinsic differentiation potential, and multi-gene expression profile, although the pXEN cell profile correlates better with the blastocyst stage. Finally, we show that pXEN cells easily convert into XEN-like cells but not vice versa. The findings indicate that (i) pXEN cells are more representative than XEN cells of the blastocyst stage; (ii) mouse pXEN, rather than XEN, cells are homologs of rat XENP cells/HypoSCs, which we propose to call rat pXEN cells. Keywords: Stem cells, Blastocyst, Mice, Rats, Extraembryonic endodermhttp://www.sciencedirect.com/science/article/pii/S1873506118301260 |
| spellingShingle | Yixiang Zhong Taewoong Choi Minjae Kim Kyoung Hwa Jung Young Gyu Chai Bert Binas Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines Stem Cell Research |
| title | Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines |
| title_full | Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines |
| title_fullStr | Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines |
| title_full_unstemmed | Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines |
| title_short | Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines |
| title_sort | isolation of primitive mouse extraembryonic endoderm pxen stem cell lines |
| url | http://www.sciencedirect.com/science/article/pii/S1873506118301260 |
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