Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of <i>Mentha</i> sp.
A microwave-assisted extraction (MAE) procedure has been optimized to simultaneously provide multifunctional extracts of <i>Mentha</i> sp. leaves with improved antioxidant properties and, for the first time, with optimal antimicrobial activity. Among the solvents evaluated, water was sel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/10/2039 |
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| author | María J. García-Sarrió María L. Sanz Jesús Palá-Paúl Silvia Díaz Ana C. Soria |
| author_facet | María J. García-Sarrió María L. Sanz Jesús Palá-Paúl Silvia Díaz Ana C. Soria |
| author_sort | María J. García-Sarrió |
| collection | DOAJ |
| description | A microwave-assisted extraction (MAE) procedure has been optimized to simultaneously provide multifunctional extracts of <i>Mentha</i> sp. leaves with improved antioxidant properties and, for the first time, with optimal antimicrobial activity. Among the solvents evaluated, water was selected as the extractant in order to develop a green procedure and also for its improved bioactive properties (higher TPC and <i>Staphylococcus aureus</i> inhibition halo). MAE operating conditions were optimized by means of a 3-level factorial experimental design (100 °C, 14.7 min, 1 g of dry leaves/12 mL of water and 1 extraction cycle), and further applied to the extraction of bioactives from 6 different <i>Mentha</i> species. A comparative LC-Q MS and LC-QToF MS analysis of these MAE extracts was carried out for the first time in a single study, allowing the characterization of up to 40 phenolics and the quantitation of the most abundant. Antioxidant, antimicrobial (<i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Salmonella typhimurium</i>) and antifungal (<i>Candida albicans</i>) activities of MAE extracts depended on the <i>Mentha</i> species considered. In conclusion, the new MAE method developed here is shown as a green and efficient approach to provide multifunctional <i>Mentha</i> sp. extracts with an added value as natural food preservatives. |
| first_indexed | 2024-03-11T03:43:31Z |
| format | Article |
| id | doaj.art-4fbb14b323cd46588230c828ed221923 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T03:43:31Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-4fbb14b323cd46588230c828ed2219232023-11-18T01:22:06ZengMDPI AGFoods2304-81582023-05-011210203910.3390/foods12102039Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of <i>Mentha</i> sp.María J. García-Sarrió0María L. Sanz1Jesús Palá-Paúl2Silvia Díaz3Ana C. Soria4Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, SpainInstituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, SpainDepartamento de Biodiversidad, Ecología y Evolución, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Genética, Fisiología y Microbiología, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, 28040 Madrid, SpainInstituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, SpainA microwave-assisted extraction (MAE) procedure has been optimized to simultaneously provide multifunctional extracts of <i>Mentha</i> sp. leaves with improved antioxidant properties and, for the first time, with optimal antimicrobial activity. Among the solvents evaluated, water was selected as the extractant in order to develop a green procedure and also for its improved bioactive properties (higher TPC and <i>Staphylococcus aureus</i> inhibition halo). MAE operating conditions were optimized by means of a 3-level factorial experimental design (100 °C, 14.7 min, 1 g of dry leaves/12 mL of water and 1 extraction cycle), and further applied to the extraction of bioactives from 6 different <i>Mentha</i> species. A comparative LC-Q MS and LC-QToF MS analysis of these MAE extracts was carried out for the first time in a single study, allowing the characterization of up to 40 phenolics and the quantitation of the most abundant. Antioxidant, antimicrobial (<i>Staphylococcus aureus</i>, <i>Escherichia coli</i> and <i>Salmonella typhimurium</i>) and antifungal (<i>Candida albicans</i>) activities of MAE extracts depended on the <i>Mentha</i> species considered. In conclusion, the new MAE method developed here is shown as a green and efficient approach to provide multifunctional <i>Mentha</i> sp. extracts with an added value as natural food preservatives.https://www.mdpi.com/2304-8158/12/10/2039<i>Mentha</i> sp.microwave assisted extraction (MAE)multifunctional extractsphenolicsliquid chromatography-mass spectrometry (LC-MS) |
| spellingShingle | María J. García-Sarrió María L. Sanz Jesús Palá-Paúl Silvia Díaz Ana C. Soria Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of <i>Mentha</i> sp. Foods <i>Mentha</i> sp. microwave assisted extraction (MAE) multifunctional extracts phenolics liquid chromatography-mass spectrometry (LC-MS) |
| title | Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of <i>Mentha</i> sp. |
| title_full | Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of <i>Mentha</i> sp. |
| title_fullStr | Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of <i>Mentha</i> sp. |
| title_full_unstemmed | Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of <i>Mentha</i> sp. |
| title_short | Optimization of a Green Microwave-Assisted Extraction Method to Obtain Multifunctional Extracts of <i>Mentha</i> sp. |
| title_sort | optimization of a green microwave assisted extraction method to obtain multifunctional extracts of i mentha i sp |
| topic | <i>Mentha</i> sp. microwave assisted extraction (MAE) multifunctional extracts phenolics liquid chromatography-mass spectrometry (LC-MS) |
| url | https://www.mdpi.com/2304-8158/12/10/2039 |
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