Robust Normalization of Luciferase Reporter Data

Transient Luciferase reporter assays are widely used in the study of gene regulation and intracellular cell signaling. In order to control for sample-to-sample variation in luminescence arising from variability in transfection efficiency and other sources, an internal control reporter is co-transfec...

Full description

Bibliographic Details
Main Authors: Andrea Repele, Manu
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/2/3/62