A colorimetric method to measure in vitro nitrogenase functionality for engineering nitrogen fixation

Abstract Biological nitrogen fixation (BNF) is the reduction of N2 into NH3 in a group of prokaryotes by an extremely O2-sensitive protein complex called nitrogenase. Transfer of the BNF pathway directly into plants, rather than by association with microorganisms, could generate crops that are less...

Full description

Bibliographic Details
Main Authors: Lucía Payá-Tormo, Diana Coroian, Silvia Martín-Muñoz, Artavazd Badalyan, Robert T. Green, Marcel Veldhuizen, Xi Jiang, Gema López-Torrejón, Janneke Balk, Lance C. Seefeldt, Stefan Burén, Luis M. Rubio
Format: Article
Language:English
Published: Nature Portfolio 2022-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-14453-x