Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease
Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a...
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Format: | Article |
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Elsevier
2005-02-01
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Series: | Neurobiology of Disease |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996104001950 |
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author | Kunjan R. Dave Ami P. Raval Jesús Purroy Ilias G. Kirkinezos Carlos T. Moraes Walter G. Bradley Miguel A. Pérez-Pinzón |
author_facet | Kunjan R. Dave Ami P. Raval Jesús Purroy Ilias G. Kirkinezos Carlos T. Moraes Walter G. Bradley Miguel A. Pérez-Pinzón |
author_sort | Kunjan R. Dave |
collection | DOAJ |
description | Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a model of motor neuron disease). The level of δPKC in the soluble fraction was significantly decreased in the spinal cord of Wobbler mice, which was associated with a significant increase in δPKC cleavage. Since caspase-3 is known to cleave δPKC, we determined caspase-3 activation in the Wobbler mice spinal cord, immunohistochemically. The results demonstrated intense immunoreactivity for activated caspase-3 in corticospinal tract motor neurons of Wobbler mice spinal cord. We hypothesize from these results that caspase-3 activation cleaves δPKC, which in turn promotes an aberrant signal transduction pathway in the Wobbler spinal cord. |
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id | doaj.art-4fe0b2119d044d27be734cb168c62a6a |
institution | Directory Open Access Journal |
issn | 1095-953X |
language | English |
last_indexed | 2024-12-22T06:12:34Z |
publishDate | 2005-02-01 |
publisher | Elsevier |
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series | Neurobiology of Disease |
spelling | doaj.art-4fe0b2119d044d27be734cb168c62a6a2022-12-21T18:36:12ZengElsevierNeurobiology of Disease1095-953X2005-02-01181126133Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron diseaseKunjan R. Dave0Ami P. Raval1Jesús Purroy2Ilias G. Kirkinezos3Carlos T. Moraes4Walter G. Bradley5Miguel A. Pérez-Pinzón6Department of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USA; Department of Neuroscience, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USA; Department of Neuroscience, University of Miami School of Medicine, Miami, FL 33101, USA; Corresponding author. Department of Neurology (D4-5), PO Box 016960, University of Miami School of Medicine, Miami, FL 33101, USA. Fax: +1 305 243 5830.Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a model of motor neuron disease). The level of δPKC in the soluble fraction was significantly decreased in the spinal cord of Wobbler mice, which was associated with a significant increase in δPKC cleavage. Since caspase-3 is known to cleave δPKC, we determined caspase-3 activation in the Wobbler mice spinal cord, immunohistochemically. The results demonstrated intense immunoreactivity for activated caspase-3 in corticospinal tract motor neurons of Wobbler mice spinal cord. We hypothesize from these results that caspase-3 activation cleaves δPKC, which in turn promotes an aberrant signal transduction pathway in the Wobbler spinal cord.http://www.sciencedirect.com/science/article/pii/S0969996104001950Amyotrophic lateral sclerosisNeuronal deathApoptosisCytochrome cNeurological disordersImmunohistochemistry |
spellingShingle | Kunjan R. Dave Ami P. Raval Jesús Purroy Ilias G. Kirkinezos Carlos T. Moraes Walter G. Bradley Miguel A. Pérez-Pinzón Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease Neurobiology of Disease Amyotrophic lateral sclerosis Neuronal death Apoptosis Cytochrome c Neurological disorders Immunohistochemistry |
title | Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease |
title_full | Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease |
title_fullStr | Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease |
title_full_unstemmed | Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease |
title_short | Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease |
title_sort | aberrant δpkc activation in the spinal cord of wobbler mouse a model of motor neuron disease |
topic | Amyotrophic lateral sclerosis Neuronal death Apoptosis Cytochrome c Neurological disorders Immunohistochemistry |
url | http://www.sciencedirect.com/science/article/pii/S0969996104001950 |
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