Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease

Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a...

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Main Authors: Kunjan R. Dave, Ami P. Raval, Jesús Purroy, Ilias G. Kirkinezos, Carlos T. Moraes, Walter G. Bradley, Miguel A. Pérez-Pinzón
Format: Article
Language:English
Published: Elsevier 2005-02-01
Series:Neurobiology of Disease
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0969996104001950
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author Kunjan R. Dave
Ami P. Raval
Jesús Purroy
Ilias G. Kirkinezos
Carlos T. Moraes
Walter G. Bradley
Miguel A. Pérez-Pinzón
author_facet Kunjan R. Dave
Ami P. Raval
Jesús Purroy
Ilias G. Kirkinezos
Carlos T. Moraes
Walter G. Bradley
Miguel A. Pérez-Pinzón
author_sort Kunjan R. Dave
collection DOAJ
description Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a model of motor neuron disease). The level of δPKC in the soluble fraction was significantly decreased in the spinal cord of Wobbler mice, which was associated with a significant increase in δPKC cleavage. Since caspase-3 is known to cleave δPKC, we determined caspase-3 activation in the Wobbler mice spinal cord, immunohistochemically. The results demonstrated intense immunoreactivity for activated caspase-3 in corticospinal tract motor neurons of Wobbler mice spinal cord. We hypothesize from these results that caspase-3 activation cleaves δPKC, which in turn promotes an aberrant signal transduction pathway in the Wobbler spinal cord.
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spelling doaj.art-4fe0b2119d044d27be734cb168c62a6a2022-12-21T18:36:12ZengElsevierNeurobiology of Disease1095-953X2005-02-01181126133Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron diseaseKunjan R. Dave0Ami P. Raval1Jesús Purroy2Ilias G. Kirkinezos3Carlos T. Moraes4Walter G. Bradley5Miguel A. Pérez-Pinzón6Department of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USA; Department of Neuroscience, University of Miami School of Medicine, Miami, FL 33101, USADepartment of Neurology, University of Miami School of Medicine, Miami, FL 33101, USA; Department of Neuroscience, University of Miami School of Medicine, Miami, FL 33101, USA; Corresponding author. Department of Neurology (D4-5), PO Box 016960, University of Miami School of Medicine, Miami, FL 33101, USA. Fax: +1 305 243 5830.Protein kinase C (PKC) was suggested to play a role in the pathology of amyotrophic lateral sclerosis (ALS) patients. Activation of PKC delta (δPKC) modulates mitochondrially induced apoptosis. The goal of the present study was to define whether δPKC activation occurs in Wobbler mouse spinal cord (a model of motor neuron disease). The level of δPKC in the soluble fraction was significantly decreased in the spinal cord of Wobbler mice, which was associated with a significant increase in δPKC cleavage. Since caspase-3 is known to cleave δPKC, we determined caspase-3 activation in the Wobbler mice spinal cord, immunohistochemically. The results demonstrated intense immunoreactivity for activated caspase-3 in corticospinal tract motor neurons of Wobbler mice spinal cord. We hypothesize from these results that caspase-3 activation cleaves δPKC, which in turn promotes an aberrant signal transduction pathway in the Wobbler spinal cord.http://www.sciencedirect.com/science/article/pii/S0969996104001950Amyotrophic lateral sclerosisNeuronal deathApoptosisCytochrome cNeurological disordersImmunohistochemistry
spellingShingle Kunjan R. Dave
Ami P. Raval
Jesús Purroy
Ilias G. Kirkinezos
Carlos T. Moraes
Walter G. Bradley
Miguel A. Pérez-Pinzón
Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease
Neurobiology of Disease
Amyotrophic lateral sclerosis
Neuronal death
Apoptosis
Cytochrome c
Neurological disorders
Immunohistochemistry
title Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease
title_full Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease
title_fullStr Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease
title_full_unstemmed Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease
title_short Aberrant δPKC activation in the spinal cord of Wobbler mouse: a model of motor neuron disease
title_sort aberrant δpkc activation in the spinal cord of wobbler mouse a model of motor neuron disease
topic Amyotrophic lateral sclerosis
Neuronal death
Apoptosis
Cytochrome c
Neurological disorders
Immunohistochemistry
url http://www.sciencedirect.com/science/article/pii/S0969996104001950
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