Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF)

Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF)

Purpose: The objective of this study was construction of recombinant hEGF-pPIC9 which may be used for expression of recombinant hEGF in following studies. Methods: EGF cDNA was purchased from Genecopoeia Company and used for PCR amplification. Prior to ligation, the PCR product and pPIC9 vector was...

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Bibliographic Details
Main Authors: Jamal Mohammadian, Sima Mansoori-Derakhshan, Masood Mohammadian, Mahmoud Shekari-Khaniani
Format: Article
Language:English
Published: Tabriz University of Medical Sciences 2013-08-01
Series:Advanced Pharmaceutical Bulletin
Subjects:
Epidermal Growth Factor
pPIC9
Polymerase Chain Reaction
Cloning
Expression
Sequencing
Online Access:http://journals.tbzmed.ac.ir/PDF/APB/Manuscript/APB-3-473.pdf

Internet

http://journals.tbzmed.ac.ir/PDF/APB/Manuscript/APB-3-473.pdf

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